【作者】 尚爱芹； 赵梁军； 田颖川；

【Author】 SHANG Ai-qin~1 ZHAO Liang-jun~2 TIAN Ying-chuan~3 (~1 College of Horticulture,Hebei Agricultural University,Baoding 071001; ~2 Department of Ornamental Horticulture and Landscape Architecture,China Agriculture University,Beijing 100094; ~3 Institute of Microbiology,the Chinese Academy of Sciences,Beijing 100080)

【机构】 河北农业大学园艺学院； 中国农业大学观赏园艺与园林系； 中国科学院微生物研究所；

【摘要】 本研究采用农杆菌介导法,以粗枝大叶黄杨无菌苗下胚轴为外植体,将含有雪花莲外源凝集素(Ga- lanthus nivalis agglutinin,GNA)基因导入粗枝大叶黄杨的下胚轴切段中。研究了抗生素种类、菌液浓度、浸染时间、选择方式等因素对粗枝大叶黄杨转化效率的影响。结果表明:对不定芽分化来说,羧苄青霉素效果 (Carb)效果明显好于头孢霉素(Cef);菌液浓度、侵染时间以及选择方式等对转化率均有较大的影响。更多还原

【Abstract】 Agrobacterium-mediated gene transformation is the mostwidely used technique for gene transferring in plants.The vector with GNA gene was transformed into the hypocotyl explants of Euonymus japonicas‘Cu zhi’.In this study the factors such as type of antibiotics,concentration of Agrohacterium,infection time and the way of selection of transformed plants were studied.The results showd that carbonicillin is superior to cefotamime for shoot differentiation.Concentration of Agrobacterium, infection time and the way of selection of transformed plants had significantly influence to the rate of transformation of Euonymus japonicus‘Cu zhi’.更多还原