四种李属红叶树种叶片花青苷提取及稳定性研究

【作者】 王庆菊； 吕福梅； 沈向；

【机构】 山东农业大学园艺科学与工程学院；

【摘要】 以两年生的紫叶桃(Prunus persica f．alropurpurea)、紫叶李(P．cerasifera ‘pissardii’)、美人梅(P．×bliriana‘Meirenmdi’)及紫叶矮樱(P．×cistena‘pissardii’)嫁接苗为试材,以油蟠桃(Prunus persica var．compressa)嫁接苗为对照,对其叶片中的色素进行提取,并进行色素的稳定性研究。结果表明,花青苷提取效果较好的溶剂为95％乙醇: 1．5mol／L HCl=85:15(V／V)。花青苷在光下不稳定,光照越强,分解越快。色素提取液中添加不同的金属离子,其影响不同,其中Fe3+、Al3+对花青素苷的增色的作用较明显,使其光密度增加13．1％～85．6％。Cu2+对叶绿素有较大影响,吸光度增加29％-38．7％。更多还原

【Abstract】 The method of pigment extraction and the stability of anthocyanins were studied in this paper using four colored Prunus taxa, P. persica f. alropurpurea, P. cerasifera ’pis-sardii’, P. × bliriana ’ Meirenmdi’, P. X cistena ’ pissardii’, P. persica var. compressa, a green leaf variety, was included as the control. The result indicated the better solvent for anthocyanins extraction was 95% ethanol : 1. 5mol/L HC1=85 : 15 (v/v) . In addition, the extractions of the leaves were treated with light of different intensity and some metal ion to test the stability of the pigments. The result is that the pigments are unstable under light, the stronger the light is, the more quickly they decompose. In the effect of metal ion, Al3+ and Fe3+ can improve the absorbance of anthocyanins about 13. 1%-85. 6%; Cu2+ can improve the absorbance of chlorophyll about 29%~38. 7%.更多还原