【作者】 朱根发； 叶秀斌； 陈明莉； 王碧青；

【Author】 ZHU Gen-fa YE Xiu-bin CHEN Ming-li WANG Bi-qing (Institute of Flower and Plant,Guangdong Agriculture Science,Guangzhou 510640,Guangdong,China)

【机构】 广东省农科院花卉研究所；

【摘要】 以墨兰企剑白墨为材料,设计2组L₉（3⁴）正交试验,研究了NH₄NO₃、KH₂PO₄、6-BA、NAA、AgNO₃、GA₃等因素对墨兰根状茎分化成苗的影响,结果表明:墨兰根状茎诱导分化出苗需要较高浓度的6-BA,以10mg/L 较为适宜;MS 培养基的NH₄NO₃常规含量比较有利于墨兰根状茎分化出苗,1/2倍MS 含量的NH₄NO₃则有利于根状茎的增殖;将培养基中的KH₂PO₄浓度提高到340mg/L,可促进墨兰根状茎的成苗;AgNO₃对墨兰的分化成苗有一定的促进作用;培养基中添加GA₃不利于墨兰根状茎的分化出苗。更多还原

【Abstract】 Effects on the plant regeneration of rhizomes from Cymbidium sinense cv.Qijianbaimo were investigated by 2 L₉（3⁴,4 factorsand 3 levels）experiments with NH₄NO₃,KH₂PO₄,6-BA,NAA,AgNO₃ and GA₃ in MS medium.The results indicate that higher concentrationof 6-BA（10mg/L）is needed for differentiation of rhizomes;that the normal content of NH₄NO₃ and 340mg/L KH₂PO₄ in MS medium is favor-able to gaining more plantlets,but half content of NH₄NO₃ is proper to promoting the propagation of rhizomes;and that AgNO₃ has some effectson the plant regeneration but adding GA₃ in MS medium is disadvantageous to the plant regeneration of rhizomes.更多还原