【作者】 张志忠； 吴菁华； 吕柳新； 何承坤； 黄碧琦；

【Author】 Zhang zhizhong, Wujing-hua, Lv liu-xin, He cheng-kun, Huang biqi(College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, Fujian 50002, China;College of life science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002,China)

【机构】 福建农林大学园艺学院； 福建农林大学生命科学学院；

【摘要】 本试验构建了以CaMV35s启动子驱动的含有番茄酸式胞外几丁质酶基因(CHI3)的植物表达载体,通过冻融法导入根癌农杆菌(Agrobacterium tumefaciens)菌株EHA105中,用叶盘法转化西瓜(Citrullus lanatus(Thunb.)Mansfeld var.citroides)栽培种“中育1号”,通过卡那霉素抗性鉴定、CHI3基因的PCR检测、Southern斑点杂交、RT-PCR检测证实CHI3基因已经整合到西瓜基因组中,并获得表达。更多还原

【Abstract】 The expression vector p121CHI3 carried tomato acidic intracellular chitinase gene(CHI3) under the drive of CaMV35s promoter, was successfully transferred into watermelon cultivars "ZhongYuYiHao" via Agrobacterium-mediated transformation. Transgenic watermelon plants were confirmed by the Kanamycin-resistant experiment, PCR analysis , Dot blotting and RT-PCR.更多还原