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番木瓜外植体消毒与继代培养的研究
A Study on Explants Sterlizing and Subculture of Papaya
【Author】 Chen Jian, You Kaizhe, Lin Guanxiong, Chen Chunfeng, and Li Weihong ( Guangzhou Fruit Research Institute , Guangzhou 510405)
【机构】 广州市果树科学研究所;
【摘要】 以“美中红”番木瓜茎尖和侧芽为材料,研究番木瓜不同外植体较适合的消毒方法,利用正交设计方法[L9(34)]研究不同培养基对番木瓜继代培养的影响,结果表明最佳继代培养基为3/2 MS+6-BA 0.5mg·L-1+GA30.2 mg·L-1+蔗糖4%,增殖系数为4.7。本研究为番木瓜良种的繁育和种质的保存提供依据。
【Abstract】 stem tips and lateral buds of Carica Papaya L. cv. Meizhonghong were used as materials in this study, and suitable sterilizing method of different explanls was found; Effects of different medium on subculture of papaya was studied by using orthogonal design [ L9 (34) ] . The results showed that the best subculture medium was 3/2MS + 6 - BA0.5mg·L-1+ GA30.2 mg·L-1+ Su4% , the coefficient of multiplication was 4.7. The study provides a method for propagation and germplasm reservation of excellent papaya cultivar.
【Key words】 Papaya; Explants sterilization; Orthogonal design; Subculture;
- 【会议录名称】 中国园艺学会第五届青年学术讨论会论文集
- 【会议名称】中国园艺学会第五届青年学术讨论会
- 【会议时间】2002-07
- 【会议地点】中国广东广州
- 【分类号】S667.9
- 【主办单位】中国园艺学会