【作者】 刘德兵； 魏军亚； 卢业凌； 李绍鹏； 秦岭； 范崇辉；

【Author】 Liu Debing, Wei Junya, Lu Yeling, Li Shaopeng, Qin Ling, and Fan Chonghui (Horticultural College, South China University of Tropical Agriculture , Danzhou , Hainan 571737; Life Science College, Zhongshan University, Guangzhou 510275 ; Horticulture Department, Beijing Agricultural College, Beijing 102206; Northwest Sci - Tech University of Agriculture & Forestry, Yangling, Shanxi 712100)

【机构】 华南热带农业大学园艺学院； 中山大学生命科学学院； 北京农学院园艺系； 西北农林科技大学；

【摘要】 通过用栗疫病菌(Cryphonectria parasitica)的菌块、病菌液体培养后滤液(CFE)、菌丝体提取物诱导剂(ME)等处理对板栗实生苗和嫁接苗(怀黄)进行诱导,测定板栗几丁质酶、β -1,3-葡聚糖酶、过氧化物酶(POD)和苯丙氨酸解氨酶(PAL)的酶活性和比活性。结果表明: 菌块处理对板栗4种酶的活性、比活性的诱导效果最佳;酶活性在接种后的35h～40h达到高峰。板栗几丁质酶具有内切酶和外切酶两种形式,以内切酶活性稍高。抗病性强的板栗实生苗酶活性高于抗病性弱的嫁接苗。更多还原

【Abstract】 The resistant chestnut seedlings and sensitive chestnut cultivars "Huai huang" to chestnut blight were selected as experimental materials. The sticks exposed to the attack of the block of Cryphonectric parasitica isolates, CFE and ME especially . The activities and specific activity of chitinase, β-1, 3 - Glucanase, POD and PAL were measured every four hours later. The results showed that the block of Cryphonectric parasitica had the highest induced effect to activity and specific activity of four enzymes. The activities of chitinase, β-1, 3 - Glucanase, POD and PAL were gradually increased and reached the top in 35 - 40hours. Endochitinase activity and indochitinase activity of chestnut were observed simultaneously, the latter was higher than the former.The activities of four enzymes of resistant seedlings were higher than that of the sensitive cultivars.更多还原