节点文献
苹果两种病毒的RT-PCR检测技术研究
Study on RT-PCR Detection Technology of Pear Ring Pattern Mosaic Virus in Kuala Pear
【Author】 Niu Jinxin and Liu Lianke ( Department of Horticulture & Forestry Engineering, Agricultural college of Xinjiang Shihezi University, Xinjiang Shihezi 832003)
【机构】 石河子大学农学院园艺园林工程系;
【摘要】 以苹果新鲜叶片和皮层为材料,改进总RNA的提取方法,获得纯度高、完整性好的总RNA,在此基础上进行了反转录和PCR扩增。检测到了苹果褪绿叶斑病毒(ACLSV)和苹果茎痘病毒(ASPV),建立了ACLSV、ASPV的RT-PCR检测程序,并且得到应用。
【Abstract】 In order to obtain high purity and integrality total RNA from fresh leaf and bark of apple infected with apple chlorotic leaf spot vims (ACLSV) and apple stem pitting virus (ASPV) , the extraction was improved. PVP powder was added to when leaf or bark was powered in order to eliminate hydroxyben-zene, and CTAB/NaCl solution was used to deposit amylose. Using total RNA as template, reverse tran-scriptase - polymerase reaction (RT - PCR) was performed. PCR amplification conditions were optimized, and the optimum RT - PCR systems of two viruses were developed. A 358bp fragment of ACLSV was obtained based on the optimum system and a 316bp fragment of ASPV.
- 【会议录名称】 中国园艺学会第五届青年学术讨论会论文集
- 【会议名称】中国园艺学会第五届青年学术讨论会
- 【会议时间】2002-07
- 【会议地点】中国广东广州
- 【分类号】S436.611
- 【主办单位】中国园艺学会