【作者】 董淑英； 赵翔宇； 陈振德； 李梅； 孙静；

【Author】 Dong Shuying Zhao Xiangyu Chen Zhende Li Mei Sun Jing (Qingdao Institute Of Agricultural Science,Qingdao 266100)

【机构】 青岛市农业科学研究所； 东农业大学；

【摘要】 为适应脱毒种薯工厂化生产的要求,降低成本,本文对马铃薯脱毒试管苗快繁方式进行了研究,在不影响脱毒苗生长的前提下,筛选出成本相对较低,增殖好的快繁方式。研究结果表明:(1)液体培养由于省去了价格昂贵的琼脂,培养成本降低了80.03%,并且试管苗的生长比固体培养要好;(2)在液体培养过程中,不加有机成分,培养成本降低23.71%,脱毒试管苗生长正常;(3)液体培养基的用量要少于固体培养基的用量,为固体培养基1/2～1/3,一般以10ml为宜。更多还原

【Abstract】 In order to apply to the demand of factory production of virus-free mini-tuber to reduce the cost, this article studies the propagation pattern of the potato virus-free tuber-plant. Under the premise of non-affecting the growth of virus-free tuber plant, we select out the propagation pattern with the lower cost and better reproduction.The result shows: (1)Without the expensive agar, using liquid culture can reduce the cost by 80.03% and gain better growth than solid culture. (2)In the liquid culture,if we eliminated the organic composition, we can reduce the cost by 23.71%, but get the normal growth. (3 )The volume of liquid culture medium is less than the solid culture medium and only 1/2 -1/3 of the latter, that is, 10ml is better.更多还原