【作者】 张延国；

【Author】 Zhang yanguo (Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081)

【机构】 中国农业科学院蔬菜花卉研究所；

【摘要】 本试验对山东莱芜大姜进行了试管姜诱导的研究。通过研究两种基本培养基、蔗糖、NAA(α-萘乙酸)、6-BA(6-苄基腺嘌呤)和光照条件对试管姜诱导的影响,成功地用组织培养的方法,由生姜脱毒组培苗诱导出微型试管姜块。主要结果如下:MS培养基比B5培养基诱导试管姜的效果好;提高培养基中的蔗糖浓度达8.0%～10.0%时,试管姜的诱导效果最佳;NAA和6-BA对试管姜形成都不起促进作用;24小时光照条件对诱导的促进作用较好。用组织培养的方法由生姜脱毒试管苗诱导形成试管姜,可以加速生姜脱毒苗应用到生产中的进程,为今后的深入研究与推广奠定了基础。更多还原

【Abstract】 In this experiment,micro-ginger induction from Shandong LaiwuDajiang were studied. Through studying the influence factors on inducing micro-ginger, such as two kinds of base medium and sucrose, NAA, 6-BA concentration in the medium and illumination condition, microrhizoms in vitro from ginger virus-free tissue culture seedlings were successfully induced.The main results are: B5 medium was less effective than MS medium. 8% - 10% surcrose concentration was best for induction. NAA and 6-BA had no promotion effect on microrhizom formation. 24hr illumination had a good effect on the induction.Success in induction of micro-ginger from virus-free plantlet in vitro can accelerate the process of application, also provide foundation for research and extension.更多还原