【作者】 徐宁生； 杨建国； 陈薇； 寸守铣；

【Author】 Xu Ningsheng Yang Jianguo (Oil Crop Research Institute, Yunnan Academy of Agricultural Sciences, Kunming 650205)Chen Wei Cun Shouxian (Biotechnology Research Institute, Yunnan Academy of Agricultural Sciences,Kunming 650223)

【机构】 云南省农业科学院油料作物研究所； 云南省农业科学院生物技术研究所；

【摘要】 本文对5个在云南种植的蓖麻品种的茎尖培养的结果进行报道。我们发现基因型在蓖麻茎尖培养中的影响比较大,品种CSR-6.190各方面的表现较佳:幼芽的增殖和伸长以及幼苗的生根表现都不错,较易获得大量幼苗,可作为用来转导外源基因的受体材料。在蓖麻的茎尖培养中,幼芽伸长是关键的一步,添加0.2mg/L 6-BA,0.01mg/L IAA或0.2mg/L 6-BA,0.01mg/L IBA的MS培养基对蓖麻幼芽的伸长有较好的效果。更多还原

【Abstract】 The meristematic explants of 5 castor (Ricinus communis ) varities planted in Yunnan have been cultured for in vitro propagation. We found genotypic differences for elongation and rooting of in vitro propagation of castor( Ricinus communis) were significant. Variety CSR-6.190 was found superior to other four varieties for obtaining highest number of plantlets from meristematic explants,which can be using in modem gene transfer technologies for plant improvement. The MS medium supplemented with 0.2mg/L 6-BA,0.01mg/L IAA or 0.2mg/L 6-BA,0.01mg/L IBA were found promising effect in elongation, which is a key step of in vitro propagation of castor( Ricinus communis ) from meristematic explants.更多还原