【作者】 许勇； 张海英； 康国斌； 范敏； 王永健； 陈杭；

【Author】 Xu Yong Zhang Haiying Kang Guobin Fan Min Wang Yongjian Chen Hang(National Engineering Research Center for Vegetable, Beijing 100089)

【机构】 国家蔬菜工程技术研究中心；

【摘要】 运用随机扩增多态性DNA（Random amplified polymorphic DNA,RAPD）与简单重复序列（Simple sequence repeat,SSR）技术,采用混合分组分析（bulked segregant analysis,BSA）方法进行了西瓜野生种质（Citrullus lanatus var.citroides）PI296341抗枯萎病基因连锁的分子标记研究.采用浸根法对西瓜抗病材料PI296341与感病材料97103,及其F₂群体105个单株进行苗期抗枯萎病生理小种1鉴定,构建了抗病基因池与感病基因池,通过RAPD与SSR多态性分析,找到了一个分子连锁标记OPP01/700与抗病基因连锁,其遗传距离为3.0厘摩（centimorgan,cM）.更多还原

【Abstract】 Random Amplified Polymorphic DNA （RAPD） and Simple Sequence Repeat （SSR） was employed as tools to detect molecular markers linked to Fusarium wilt resistant gene in the watermelon wild germplasm （Citrullus lanatus var. citroides）. From screening the Fusarium wilt resistance of F₂ population consisted by the introgression of Fusarium wilt resistant gene from PI296341 into the susceptible inbred 97103 by using root- dip inoculation, the pools of Fusarium wilt resistance and susceptibility were estabhshed. Screening the repeatable polymorphism between paired bulks, OPP01/700 was proved to be linked to the resistant gene by co - segregation analysis with F₂ population. The genetic distance is 3.0 centimorgan（cM）₀ .更多还原