【作者】 陈昆松； 李方； 张上隆； GavinS.Ross；

【Author】 Chen Kunsong Li Fang Zhang Shanglong Gavin S. Ross(Lab of Fruit Molecular Physiology, Department of Horticulture, Zhejiang University, Huajiachi Campus, Hangzhou 310029, P. R. China)(The Horticulture and Food Research Institute of New Zealand Ltd. , Mt. Albert Research Center, Private Bag 92 169, Auckland, New Zealand)

【机构】 浙江大学园艺系果实分子生理学实验室； 新西兰国家研究院园艺与食品研究所；

【摘要】 本试验从成熟中华猕猴桃果实中克隆到了一个74bp的β-半乳糖苷酶基因cD-NA片段,与苹果、芦笋、绿花椰菜、番茄中β-半乳糖苷酶基因cDNA相应区段的核苷酸同源性为67.3%～70.3%,氨基酸同源性为69.1%～72.7%,用该片段为探针进行Northern分析表明,果实采收时,β-半乳糖苷酶mRNA水平最高,随后呈下降变化,同时β-半乳糖苷酶基因的表达可为外源乙烯所诱导,但在果实乙烯跃变期间β-半乳糖苷酶基因的表达信号无显著变化.文中对β-半乳糖苷酶在猕猴桃果实成熟衰老过程中的作用进行了讨论.更多还原

【Abstract】 We cloned an 747 bp long cDNA fragment encoding part of theβ - galactosi- dase protein by PCR from ripening Actinidia chinensis fruit. Comparisons between the βgalactosidase sequence from kiwifruit and others from apple, asparagus, broccoli and tomato indicated nucleic acid homology between 67.3% and 70.3% , and amino acid identity be-tween 69. 1 % and 72.7% . Northern analysis showed thatβ- galactosidase mRNA was abundant in the fruit at harvest time and then went down. The mRNA level was the lowest at the stage of 24h ethylene treatment. A transient increase in the mRNA level was seen after 24h ethylene treatment and no significant change during ethylene climacteric in ripening fruit. The significance of these results is discussed.更多还原