【作者】 李卫东； 葛会波； 魏景芳； 张广华； 肖建忠；

【Author】 Li Wedong Ge Huibo Wei Jingfang Zhang Guanghua Xiao Jianzhong Department of Horticulture, Hebei Agricultural University Baoding 071001 Physiological and Biochemistry Institute of Hebei Agricultural Academy Shijiazhuang 053000

【机构】 河北农业大学园艺系； 河北农业科学院理化所；

【摘要】 以草毒品种宝交早生为试材,用F培养基,附加BA2.0mg/L,NAA0.2mg/L,CH300mg/L进行花药愈伤组织诱导,其出愈率达89.13%.以MS为基本培养基,通过改变培养基中的激素种类及浓度、有机附加物的种类及浓度、无机盐的种类和浓度,愈伤组织在继代培养过程中的状态不断变化,并诱导出胚性愈伤组织.胚性愈伤组织在MS无机盐+B₅有机物+2,4-D1.0mg/L+NAA1.0mg/L+ZT0.2mg/L+CH400mg/L+Gln150mg/L+KCl400mg/L+蔗糖3%（PH=5.8）的液体培养系中培养一个月,建立了细胞悬浮系,并诱导发生了胚状体.更多还原

【Abstract】 Callus formation of strawberry ( Fragaria ananassa Duch, cv: Houkouwase) was achieved using F medium added with BA2. 0mg/l, NAA0. 2mg/l, CH300mg/. The callus induction rate was 89.13% , The state of the callus was regulated by modificating the con-centration of hormones, organic and inorganic additives during subculture and embryogenic callus was gained from Houkouwase anther callus. Cell suspension was established by cul-turing embryogenic callus in liquid medium containing MS salts + B5 organic additives + 2, 4 - D1. 0 mg/L + NAA1. 0 mg/L + ZT0. 2 mg/L + CH400 mg/L + Gln150 mg/L + KCL400 mg/L + surose3 % , pH = 5.8, Embriyoids were formed from thesuspension during culturing更多还原