【作者】 申书兴； 刘学岷； 张成合； 王子欣；

【Author】 Shen Shuxing, Liu Xuemin, Zhang Chenghe, and Wang Zixin (Department of Horticulture, Hebei Agricultural University, Baoding 071001;Institute of Vegetables and Flowers, Hebei Agricutural Academy, Shijiazhuang 050051)

【机构】 河北农业大学园艺系； 河北省农业科学院花卉研究所；

【摘要】 该研究表明:以能产生2n配子的二倍体大白菜自交不亲和系Bp058或不能产生2n 配予的二倍体大白菜自交不亲和系252为亲本,分别与四倍体水仙花(SXH)进行杂交时,都能够完成授粉受精过程,但252(2x)×SXH(4x)组合,因胚乳提早解体退化,使杂种幼胚在分化前都败育死亡;Bp058(2x)×SXH(4x)组合的杂种幼胚在分化前大多数也败育死亡,有少数杂种幼胚可正常发育成成熟种子;这些杂种后代经鉴定全为四倍体;用胚胎学方法观察测定可知,二倍体 Bp0582n雌配子形成率为8．33％,其受精率为66．03％。更多还原

【Abstract】 The dipliod chinese cabbage ( Brassica campestris var. pekinensis) Bp058, which can produce 2n gametes and the inbreeding line 252 which can not produce 2n gametes were used to cross with tetraploid "shuixianhua" (SXH) in the studies of embryo genetic cause of new tetraploid hybrids. The results showed the germination of pollen grains, growth of pollen tubes, and fertilization of Bp058(2x)-SXH(4x) and 252(2x)-SXH(4x) were normal when compared with control 252(2x)-252(2x). But all the hybrid embryos of 252(2x)-SXH(4x) were aborted before differentiation. The embryo abortion was the consequence of endosperm deterioration. Most hybrid embryos of Bp058(2x)-SXH(4x) also aborted, but a few embryos could develop into mature embryos. Therefore, the new tetraploid hybrids were produced by fertilization of 2n gametes of Bp058(2x) and n gametes of SXH(4x). The frequency of 2n female gamete generation was 8. 33% by determining the development of hybrid embryos of Bp058-SXH in which Bp058 was used as the pistillate parent. The 66. 03 percent of the 2n female gametes of the diploid Bp058 could be fertilized in the cross Bp058-SXH.更多还原