【作者】 缪颖； 曹家树； 陈云鹏；

【Author】 Miao Ying, Cao Jiashu, and Chen Yunpeng (Cell and Molecular Biology Laboratory, National Key Discipline of Vegetable Science, Zhejiang Agricultural University, Hangzhou 310029)

【机构】 浙江农业大学国家蔬菜重点学科细胞与分子生物学实验室；

【摘要】 本文简要介绍几种根据不同的DNA杂交靶提高荧光原位杂交分辨率的方法,如高分辨率的染色体靶、问期核染色质靶和DNA纤丝靶、以及该技术在植物学研究中的应用现状和在园艺科学研究中的应用前景。更多还原

【Abstract】 The present paper briefly introduced the methods of high resolution visual mapping by fluorescent in situ hybridization based on differences in the preparations of target DNAs such as high resolution chromosomal targets, interplace nuclei as targets and DNA fibers as targets. Using high resolution FISH technique, it is possible to obtain simultaneous information rapidly in a convenient visual form about the order, transcriptional orientation and physical distances, separation clones, especially utilities in the construction of long range physical maps of genes of horticultural crop and the study on time and space expression of developmental-related gene.更多还原