【作者】 刘志敏； Nowak J．； Asiedu S．K．； Pillay V．； Stewart A．； Lazarovits G．； Smith C.；

【Author】 Liu Z.Nowak J., Asiedu S. K., Lazarovits G., Pillay V.. Stewart A., Smith C. (Department of Plant Science-Nova Scotia Agriucltural College-Truro, N. S. Canada B2N 5E3;Agriculture Canada Research Station 1391 Standford Street-London, ON Canada N5B 4T3;Huan Agricultural College-Changsha-Hunan-China.)

【机构】 湖南农业大学园艺系； 加拿大诺斯科舍农学院； 加拿大农业研究所；

【摘要】 利用一种从洋葱根际分离出的能促进作物生长的假单胞杆菌PsJN,无菌接种到马铃薯、番茄、辣椒、黄瓜和西甜瓜的节段和幼苗上,结果表明被接种有PsJN细菌的幼苗在根系生长、根群的发育和根重等方面明显高于对照,其差异以番茄、马铃薯表现明显,其次为辣椒、西瓜、和黄瓜, 对甜瓜的效果不甚明显。该种细菌也能明显地改进马铃薯、番茄、辣椒和黄瓜试管苗移植到温室条件下栽培的成活率和早期生长,并可大大改善试管苗对室外栽培条件的适应能力。接种有PsJN的马铃薯幼苗对低水平的Vericllium alboatrum和Rhizoctonia solani比未接种的有更好的抗逆性, 该种细菌与植株形成共生或内生的关系,并不需对外植体进行代代接种,故可在大范围生产上加以应用。结果同时表明,根际细菌在提高植物对环境胁迫的抗逆性上起着重要作用。更多还原

【Abstract】 A plant growth promoting pseudomonad bacterium, strain PsJN, isolated from surface sterilized onion roots, stimulated in vitro growth of potato , tomato , pepper, cucumber and watermelon when nodal explants or seedlings were inoculated with approximately 2. 8×108 CFU/ml bacterium suspension and grown on a hormone-free MS medium under 16h photoperiod, 120-160/μE/m2. s fluorescent light and 22/19℃ light/ night cycle. The bacterized plantlets had a better developed root system,improved shoot growth and higher biomass than the non-inoculated controls. The best responding were tomatoes and potatoes, followed by pepper , watermelon and cucumber; canaloupe did not responde. Bacterization also significantly improved greenhouse establishment and/or early growth after transplanting of potato, tomato, pepper and cucumber and field survival of potatoes transplanted directly from culture vessels. Bacterized potato plantlets were also more resistant to low levels of Verticillium alboatrum and Rhizoctonia solani than the non-inoculated controls. The bacterium can be used in large-scale plantlet production. It grows poorly on the culture medium in the absence of plant and forms endo- and epiphytic populations, thus eliminating the need for explant re-inoculation. The results indicate that rhizosphere bacteria may play an important role in plant responses to environmental stresses.更多还原