【作者】 许榕仙； 向建军；

【Author】 XU Rong-xian XIANG Jian-jun Department of Nutrition and Health Care,School of Public Health Fujian Medical University,Fuzhou 350004,China

【机构】 福建医科大学公共卫生学院营养与保健医学系；

【摘要】 目的观察叶酸（FA）、维生素 B₆（VB₆）、维生素 B₁₂(VB₁₂)对同型半胱氨酸致血管内皮细胞损伤的交互保护作用。方法原代培养大鼠主动脉内皮细胞,MTT 法观察叶酸、VB₆、VB₁₂各自单独5 个剂量（加上阴性阳性对照组,共7组）对 Hcy 抑制大鼠主动脉内皮细胞（RAEC）增殖的保护作用。然后采用 L₂₇(3¹³)正交设计,通过 MTT 细胞增殖实验观察叶酸、VB₆、VB₁₂的交互作用；根据正交分析结果分为6组,三组维生素防护组、阴性对照组、阳性对照组及维生素对照组,维生素用含终浓度为1．0mmol/ LHcy 的培养基配制,干预24h 后,取上清液,比较各组超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶 （GSH-Px）、丙二醛（MDA）、一氧化氮（NO）、一氧化氮合酶（NOS）等指标的差异。结果叶酸与 VB₁₂能够缓解1．0mmol/L Hcy 对大鼠主动脉内皮细胞（RAEC）增殖的抑制作用,而 VB₆则无明显效果；叶酸与 VB₆ 无交互作用,与 VB₁₂存在交互作用；VB₆与 VB₁₂之间也存在交互作用；FA、FA+VB₁₂、FA+VB₆+VB₁₂三种组合均能拮抗 Hcy 对 SOD、GSH-Px、NOS 酶活性的抑制作用,保护内皮细胞功能。结论同时补充叶酸、 VB₆、VB₁₂可能是三者在体外保护 Hcy 损伤内皮的较理想组合。更多还原

【Abstract】 Objective to investigate the cross protective effects of folic acid,VB₆,VB₁₂ on rat aortic endothelial cells injuried by DL-homocysteine （Hcy）.Method Rat aortic endothelial cells （BAEC） were cultured in vitro,and the cross effects of folic acid,VB₆,VB₁₂ were studied according to orthogonal design by MTT. According to the results of orthogonal design,the activities of SOD、GSH-Px、NOS and the contents of MDA、NO in the medium were compared.Results The results of orthogonal design indicate that,the inhibition of DL- homocystine to RAEC could be relieved by folic acid,VB₁₂ excluding VB₆.The interaction existed between folic acid and VB₁₂ as well as VB₆ and VB₁₂.The inhibition of Hcy to the enzymes （SOD、GSH-Px、NOS） could be relieved by three vitamin projects.Folic acid,VB₁₂,VB₆ could inhibit the reduction of NO and decrease the production of MDA.Conclusion The simultaneous addition of folic acid,VB₆ and VB₁₂ may be an excellent strategy for protection of RAEC injuried by DL-homocysteine.更多还原