【作者】 刘胜浩； 林学政； 刘晨临； 黄晓航；

【Author】 LIU Sheng-hao, LIN Xue-zheng, LIU Chen-lin, HUANG Xiao-hang (First Institute of Oceanography, SOA, Qingdao 266061, China; Key Lab of Marine Bioactive Substances, SOA, Qingdao 266061, China)

【机构】 国家海洋局第一海洋研究所；

【摘要】 极端微生物是亟待开发的微生物资源宝库。通过PCR扩增南极嗜冷菌低温脂肪酶基因序列,将其连接到表达载体pET-24a(+)中,转化大肠杆菌BL21(DE3)后,实现低温脂肪酶在常温菌的高效表达,将对低温酶的催化机制研究和工业化应用起到促进作用。更多还原

【Abstract】 Extremophiles are microbial resource treasury needing exploitation. In this paper, the cryolipase gene sequence from Antarctic psychrophilic bacteria was amplified by polymerase chain reaction (PCR), then linked to the expression vector pET-24a( + ), and the high-level expression of cryolipse in recombi-nant E. coli was realized by converting E. coli BL21(DE3), which will promote the study on catalytic reaction mechanism and the industrialized application of cryolipase.更多还原