【作者】 郑洲； 缪锦来； 丁燏； 张波涛； 李光友；

【Author】 ZHENG Zhou,MIAO Jin-lai,DING Yu,ZHANG Bo-tao, LI Guang-you (First Institute of Oceanography, SOA, Qingdao 266061, China; Ocean University of Guangdong , Zhanjiang 524025, China)

【机构】 国家海洋局第一海洋研究所； 广东海洋大学；

【摘要】 因为污染毒害会引起生物体内活性氧的增加,所以生物体抗氧化系统的变化可以作为污染毒害引起的氧化胁迫程度的标记。南极细菌优势菌株Pseudoalteromonas sp．NJ62在用氯化汞处理后的抗氧化酶和汞还原酶的活性变化实验结果表明,Pseudoalteromonas sp．NJ62的超氧化物酶主要为Mn-SOD,当用5 μmol／L和10 μmol／L Hg2+处理时,其活性增加;在用Hg2+处理后,谷胱苷肽过氧化物酶、谷胱甘肽还原酶和汞还原酶的活性也显著增加。这些抗氧化反应可以用来作为南极生态系统汞污染引起的氧化胁迫的生物标记。更多还原

【Abstract】 Because the pollutant toxin can cause the increase in active oxygen in the organisms, the variations in antioxidant systems in the organisms can be taken as the biomarkers for indicating the oxidative stress degree induced by pollutant toxin. An experiment was made to study the variations in activities of antioxidant enzymes and mercuric reductase in Antarctic bacterium Pseudoalteromonas sp. NJ62 following exposure to HgCl2. It is shown from the experimental results that the main superoxide dismutase in Pseudoalteromonas sp. NJ62 is Mn-SOD, and Mn-SOD activity increased after being treated with 5 μmol/ L and 10μmol/L Hg2+ solutions; and the significant increases in activities of glutathione peroxidase, gluta-thione reductase and mercuric reductase were observed after being treated with Hg2+ solutions, so these antioxidant responses can be used as the biomarkers for indicating oxidative stress induced by mercury pollutants in the Antarctic ecosystems.更多还原