【作者】 刘炯宇； 江建平； 何开泽； 谢锋； 张逊； 郑中华； 周洲；

【Author】 LIU Jiong-yu, JIANG Jian-ping, HE Kai-ze, XIE Feng, ZHANG Xun,ZHENG Zhong-hua, ZHOU Zhou(Chengdu Institution of Biology, the Chinese Academy of Sciences, Chengdu 610041,China;Detect Center of Chengdu Quan Xing Distillery, Chengdu 610036, China)

【机构】 中国科学院成都生物研究所； 四川全兴股份有限公司检测中心；

【摘要】 <正> 山溪鲵皮肤分泌物和经两次SephadexG-25层析分离得到的初步提纯样品经抗菌实验,发现具有抗菌活性。经胰蛋白酶消化实验证明其中的抗菌活性成分为蛋白质性质。此抗菌活性成分具有在高温下短时间、常温下长时间保持稳定的特性。初步提纯样品经尿素SDS聚丙烯酰胺凝胶电泳检测为两条带,分子量分别为19.7kD和23.0kD;经反相高效液相层析(RP-HPLC)检测为13个峰。此提纯方法的活性回收率为46.7%。这为进一步的纯化和性质鉴定工作奠定了基础。更多还原

【Abstract】 The skin secretion of Batrachuperus pinchonii and the Part Purified Substance, acquired by the skin secretion loaded on Sephadex G-25 chromatography column twice, have antimicrobial ability by antimicrobial test. The antimicrobial active substance is protein property by trypsin digest test, stable in high temperature during short time and in normal temperature during long time. By electrophoresis in urea polyacrylamide gel with sodium dodecyl sulftate, the Part Purified Substance has two bands, the molecular weight is 19.7kD and 23.0kD respectively; by RP-HPLC chromatograph, it has thirteen peaks. The recovery of activity is 46.7%. On the basis of which further works including final refining and determining of the active substance can be finished.更多还原