【作者】 耿越； 涂文利； 张健； 张静静； 张晾；

【Author】 Geng Yue, TU Wen-li, Zhang Jing-jing, Zhang Liang, Zhang Jian, Tian Bo (College of Life Science, Shandong Normal University, Jinan 250014, China )

【机构】 山东师范大学生命科学学院；

【摘要】 目的使用超临界二氧化碳萃取技术萃取油菜花粉中的脂质,研究萃取物对高脂血大鼠的降血脂效果。方法建立大鼠高脂血模型,大鼠随机分为基础饲料对照组、高脂饲料对照组、阳性药物对照组、低剂量萃取物组、中剂量萃取物组、高剂量萃取物组。药物和花粉萃取物灌胃,四周后处死大鼠,检测血清和肝脏指标。结果:油菜花粉超临界二氧化碳萃取物是以α-亚麻酸为主的不饱和脂肪酸油脂。与高脂对照组相比,不同含量的花粉萃取物可以不同程度的降低高脂血大鼠血清中TG和TC水平;降低肝脏中TC水平, 明显升高肝脏HDL-C和apoAl水平,三个剂量组对大鼠肝脏LDL-C和apoB降低效果不同,显著降低大鼠肝脏中游离胆固醇水平(P<0．05);花粉萃取物组能升高大鼠血清LCAT水平,抑制HMG-CoA还原酶活性; 花粉萃取物肝脏脂肪酸中DHA含量明显高于高脂时照组。结论:油菜花粉对高血脂大鼠血脂的降低作用, 主要是通过增加HDL-C和LCAT水平。促进胆固醇转运、代谢和排出,抑制HMG-CoA还原酶活性减少胆固醇体内合成,并提高肝脏中DHA含量起作用。更多还原

【Abstract】 Objective This study aimed to evaluate the effect of extract by supercritical carbon dioxide extraction from rape pollen on lipid metabolism in experimental hyperlipidemic rats. Methods The experimental hyperlipidemic rats were establish by high cholesterol diets. The rats were randomized into six groups including normal control group, high fat control group, medicinal control group, lo-pollen’s extraction group, mid-pollen’s extraction group, and hi-pollen’s extraction group . After four weeks of intraperitoneal injection, the rats were executed, and lipids levels of serum and hepatic tissue were detected. Result: The plasma levels of total cholesterol (TC) and triacylglycerol (TG) were significantly lower in the pollen’s extraction group and medicinal control group than in the high fat group. Hepatic TG and TC levels were decreased in rats fed pollen’s extraction and medicinal diets compared with high fat diets. A higher concentration of HDL-C and apoAI in hepatic tissue was detected after the pollen’s extraction diet compared to the high fat diet ( P < 0. 05), whereas reducing of the hepatic LDL-C and apoB concentration shows different effects a- mong the pollen’s extract diets. LCAT in serum of pollen extraction was significant high than HFC, also HMG-CoA re-ductase show decrease tendency in experimental group of pollen extraction. We can drew conclution from valuable data of fatty acid composition that DHA of hepatic lipid was high in pollen extraction group than HFC group. Conclusion These results suggest that extract from rape pollen rich in alpha-linolenic acid seem to decrease the degree of lipid in plasma and hepatic tissue compared to high fats. diet. This is possibly due to increasing activity of LCAT and decreasing activity of HMG-CoA reducase and the ratio of clearance of free cholesterol so as to promote transportation, excretion and metabolism of cholesterol in hepatic tissue and serum, also indirectly increase in DHA of heptatic tissue.更多还原