【作者】 李静； 刘颖生； 李龙；

【Author】 LI Jing, LIU Yingsheng, LI Long (Department of Health Toxicology and MOE Key Lab of Environment and Health, School of Public Health,, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030 , China)

【机构】 华中科技大学同济医学院公共卫生学院卫生毒理学系教育部环境与健康重点实验室；

【摘要】 目的:研究软骨藻酸诱导PC12细胞凋亡作用及对细胞周期和活性氧生成的影响。方法:流式细胞仪法测定0、0.01、0.1、1μmol/L软骨藻酸作用细胞24h后对细胞凋亡及细胞周期和活性氧生成的影响。结果:软骨藻酸可诱导PC12细胞凋亡的发生,所有剂量组的凋亡发生率与对照组比较,差异具有显著性。流式细胞仪法测定软骨藻酸对PC12细胞细胞周期的影响:软骨藻酸可将PC12细胞阻滞于细胞周期中的S期,中、高剂量组中处于S期的PC12细胞数量和百分比与对照组比较,差异具有显著性。软骨藻酸对PC12细胞活性氧生成的影响:软骨藻酸对PC12细胞ROS的产生具有一定的影响,高、中、低剂量组均可使PC12细胞的ROS产生量增加,与对照组相比均具有显著意义。结论:软骨藻酸能诱导PC12细胞凋亡的发生,将PC12细胞阻滞于细胞周期中的S期,并使得细胞内的ROS产生增加。更多还原

【Abstract】 Objective: To study the apoptotic PC12 cells induced by Domoic acid and the effect of Domoic acid on the cell cycle and reactive oxygen species in PC12 cells. Methods: The apoptotic PC12 cells, the cell cycle and reactive oxygen species was measured by the Flow cytometric analysis treated with 0、0.01、0.1、1μmol/L Domoic acid 24h. Results: Flow cytometric analysis indicated that the Domoic acid could induce apoptosis of PC12 cells. The effect on the cell cycle: The cell cycle was blocked markedly at S phase after exposing PC12 cells to Domoic acid for 24 h specifically in the middle and high concentrations. Reactive oxygen species (ROS) induced by Domoic acid: Domoic acid had an effect on ROS in PC12 cells, and three dosages exhibited more elevated ROS level than that of the control group. Conclusion: Domoic acid could induce apoptosis of PC12 cells, block the cell cycle at S phase and elevate ROS the level in PC12 cells.更多还原