【作者】 宋旭； 张振中；

【Author】 SONG Xu, ZHANG Zhenzhong* (Pharmacy College of Zheng Zhou University Daxue Road 40, Zhengzhou, 450052)

【机构】 郑州大学药学院；

【摘要】 采用均匀设计考察了PH值,DNA浓度,正负电荷比和离子强度对聚赖氨酸—DNA纳米缩合体粒径的影响。所得的结果为:只有正负电荷比,DNA浓度平方,与平均粒径相关。DNA浓度在14.07mM作用,正负电荷比为1.35左右。可制备得到具有最小粒径(201.783nm)的聚赖氨酸—DNA缩合体。更多还原

【Abstract】 We arrange the experiment according to uniform design table to study the influence of PH, concentration of DNA, ratio of positive charges and negative charges, ion strength to PLL-DNA condensate’s size .The result is that: Only do ratio of positive charges and negative charges, concentration of DNAcorrelate with the size of PLL-DNA condensate. When concentration of DNA is 14.07mM,ratio of positive charges and negative charges is 1.35, we can prepare PLL-DNA condensate which size is 201.783nm.更多还原