【作者】 季祥； 焦建龙； 蔡禄；

【Author】 JI Xiang, JIAO Jianlong, CAI Lu (School of Biological and Chemical Engineering, Inner Mongolia University of Science and Technology, BaoTou 014010, Inner Mongolia)

【机构】 内蒙古科技大学生物与化学工程学院；

【摘要】 从富油土壤中分离到9株产脂肪酶杆菌,其中BD3菌株产脂肪酶能力较强,通过测定其生理生化特征,初步鉴定该菌为假单胞菌属。我们利用聚乙烯醇(PVA)和硼酸交联作为固定化BD3菌株细胞的载体,并进行了转酯化的初步研究,发现低浓度PVA制成的固定化细胞首批催化效率明显高于高浓度PVA制成的固定化细胞,而且固定化细胞之后的酶活力与固定化细胞前的酶活力相比有所下降,但固定化细胞可重复使用多批次仍然有活性。更多还原

【Abstract】 9 strains producing lipase were isolated from rich oil soil. The lipase from has the highest activity to catalyze glycerolysis of oil. Strain BD3 was identified as Pseudomonas sp. BD3 was immobilized by PVA and Boric Acid, Immobilized cells were treated with deffirent concentration of PVA , We obtain the conclusions that the high activity was maintained even after many batchs.更多还原