【作者】 张国良； 陈文军； 戴其根； 许轲； 霍中洋； 张洪程；

【Author】 ZHANG Guo-liang~(1,2) CHEN Wenjun~1 DAI Qi-gen~1 XU Ke~1 HUO Zhong-yang~1 ZHANG Hong-cheng~1 (1.Key Lab for Crop Genetics and Physiology of Jiangsu Province,Yangzhou University,Yangzhou 225009,China;2.College of Life Science and Chemistry Engineering,Huaiyin Institute of Technology,Huai’an 223001,China)

【机构】 扬州大学江苏省作物遗传生理重点实验室；

【摘要】 为了评定不同水稻基因型对1,2,4-三氯苯(TCB)的耐性强弱,确定合适的筛选指标和筛选浓度,采用发芽试验和砂培水稻幼苗的方法,先以0(对照)mmol/Kg 沙、0．35 mmol/Kg 沙和3 mmol/Kg 沙3个浓度的 TCB 处理20个水稻基因型,确定0．35 mmol/Kg 沙比3 mmol/Kg 沙更为合适用于水稻基因型耐性鉴定。然后以0(对照)mmol/Kg 沙、0．35 mmol/Kg 沙2个浓度的 TCB 处理50个水稻基因型。以发芽势、发芽率、最长根长、根数、株高、地上部干重、根干重和总干重的性状相对值(处理测定值/对照测定值×100%)作为幼苗耐性指数(tolerance index, TI)。基于耐性指数对各基因型进行聚类分析,将50个水稻基因型聚为耐性、较耐、较敏感和敏感4类。更多还原

【Abstract】 1,2,4-trichlorobenzene (1,2,4-TCB) has pervaded in natural environment and industrial production.It would cause a series of problems due to its longevity and amassment.The EPA has added it into the environmental pollutant list.In order to evaluate the tolerance of the fifty rice genotypes to 1,2,4-TCB stress and identify proper screening indices and concentration,the effects of 1,2,4-TCB on the rice seed germination and seedling were studied.At first,the concentrations of 1,2,4-TCB treated 20 genotypes were 0 mmol/Kg sand,0.35 mmol/Kg sand and 3 mmol/Kg sand,and 1,2,4-TCB dissolved in 0.3 ml acetone were added into sands.The result of this experiment showed that 0.35 mmol/Kg sand was the better screening concentration.So 0 mmol/Kg sand and 0.35 mmol/Kg sand were adopted to treat 50 genotypes in the latter study.Germination potential,germination rate,the longest root length,root number,plant height,the dry weight of shoot and roots of different rice varieties at seedling stage were measured.Character relative values were got from measure values of treated groups and control groups.They were used as tolerance indices (TI) of early seedlings.Cluster analysis of different wheat varieties based on TI was adopted.The results showed that the fifty rice varieties could be grouped into four types,.i.e.TCB sensitive varieties,relatively sensitive varieties, tolerant varieties and relatively tolerant varieties.更多还原