【作者】 刘立军； 陈荷泉； 蒙祖庆； 高世梅； 彭定祥；

【Author】 LIU Li-jun CHEN He-quan MENG Zu-qing GAO Shi-mei PENG Ding-xiang (College of Plant Science & Technology,Huazhong Agricultural University,Wuhnn 430070,China)

【机构】 华中农业大学植物科学技术学院；

【摘要】 利用RAPD分子标记技术对44份苎麻栽培品种材料进行遗传多样性分析。从50个 RAPD 引物中筛选出 23个多态性好的引物构建 DNA 指纹图谱来鉴别苎麻品种的基因型。这23个 RAPD 引物获得的电泳图谱结果表明,本研究中所有供试品种能互相区分开来并表现出丰富的遗传多样性,说明 RAPD 是一种鉴定苎麻品种 (系)比较有效的方法。23个 RAPD 引物共扩增出了112条不同位置的带,平均4．86条;多态性带103条,平均 4．47条,多态性为91．7%。不同引物的多态带百分率在66．7～100之间。44份材料的遗传相似系数在0．57～ 0．99之间。经过聚类分析,可以将这些材料分为4大类,共5个亚组,并且在一定程度上与血缘关系是一致的, 也存在一些例外。结果表明。RAPD 标记尽管可以用于进行遗传多样性分析,但并不是最好的分子标记类型。综合考虑到不同的分子标记的优缺点,认为 RAPD 标记在遗传研究较少的作物上应用潜力较大。更多还原

【Abstract】 The genetic diversity of 44 Ramie cuhivars were analyzed by using RAPD markers.Of the 50 primers screened,23 primers gave reproducible,polymorphic DNA amplification patterns,and were selected to construct a DNA fingerprinting map to distinguish the genotypes of Ramie.According to the banding patterns obtained with 23 selected primers,all cultivars tested in this study were distinguished from each other and showed ample genetic diversity,indicating that RAPD-PCR was an effective meth- od for cultivar identification of ramie.With 23 RAPD primers,totally 112 alleles were detected among which 103 were polymorphic,and the average number of alleles for one primer was 4.86.Ratio of polymorphic bands to amplified bands ranged from 66.7 to 100.The similarity coefficient of 44 acces- sions ranged from 0.57 to 0.99.Cluster analysis based on RAPD data showed that the accessions could be categorized into four groups and further 5 sub-groups,to some extent in accordance with the pedigrees of the entries and with some exceptions.Therefore,the present study indicated that RAPD markers were not the best marker system for assessment of genetic diversity although it might reveal ge- netic relationships among accessions.It was suggested that RAPD be used for assessment of genetic di- versity in under-researched or neglected crops.更多还原