【作者】 牛吉山； 刘瑞； 洪德峰；

【Author】 NIU Ji-Shan LIU Rui HONG De-Feng (National Engineering and Research Center for Wheat,Henan Agricultural University,Zhengzhou 450002,China)

【机构】 河南农业大学国家小麦工程技术研究中心；

【摘要】 抗病性的提高是现代作物品种成功推广的前提条件。小麦抗病分子机理研究将为抗性改良提供理论基础。从抗白粉病分子机理研究入手,从小麦中克隆、研究了 TaMlo,TaLRK,TaEDR1,TaPR-1,TaPR-2,TaPR -5,与水稻 Xa21类似的 TaXa(暂时标记)。与拟南芥 NPR1类似的 TaNPR1(暂时标记),在小麦中检测到与大麦抗病相关的 RAR1,SGT1,HSP90等类似基因的表达产物。用转基因过量表达、反义 RNA 和 dsRNAi 技术已经将 TaLRK,TaEDR1和 NPR1基因向小麦中进行了转化,为小麦抗病信号途径的研究和抗性改良奠定了基础。更多还原

【Abstract】 Disease resistance strategies are major components of modem,sustainable agriculture. Studies on molecular mechanism of wheat disease resistance will provide theoretic bases for disease re- sistance improvement.Our study is focused on the molecular mechanism of powdery mildew resistance. We isolated and studied wheat genes,which may be associated with powdery mildew resistance reac- tion.They are TaMlo,TaLRK,TaEDR1,TaPR-1,TaPR-2,TaPR-5,TaXa(temporary symbol,simi- lar to Oryza sativa disease resistance gene Xa21)and TaNPR1(temporary symbol,similar to Arabi- dopsis thalina gene NPR1).We also detected the transcription products of the wheat genes similar to harley disease resistance related genes of BAR1,SGT1 and HSPg0.Using gene over-expression,anti- sense RNA and dsRNAi techniques,TaLRK,TaEDR1 and NPR1 genes were transferred into wheat. These transgenic plants provided bases for studies of disease resistance signal pathway and wheat dis- ease resistance improvement.This paper is a summary of our studies.更多还原