【作者】 王萍； 郭永来； 王罡； 季静； 余云舟； 李昌； 金宁一；

【Author】 WANG Ping~1 GUO Yong-lai~2 WANG Gang~3 JI jing~3 YU Yun-zhou~4 LI Chang~4 JIN Ning-yi~4 1.Ocean school,Huaihai Institute of Technology,Jiangsu Lianyungang 222005,China; 2.Changchun Longyang Soybean Biologic Albumen Co.Ltd,Jilin Changchun 130062,China; 3.Agriculture & Bioengineering College,Tianjin University,Tianjin 300072,China; 4.Veterinary Institute,Academy of Military Medical Science,Jilin Changchun 130062,China

【机构】 淮海工学院海洋学院； 长春龙洋大豆生物蛋白股份有限公司； 天津大学农业与生物工程学院； 军事医学科学院军事兽医研究所；

【摘要】 本研究以大豆体细胞胚为受体,用农杆菌介导法将口蹄疫病毒结构蛋白全长基因 P1导入大豆基因组中,获得了抗性植株。经 GUS 染色、PCR 及 PCR-southern 杂交等分子检测,证明目的基因已导入并整合到大豆基因组中,为利用大豆作为生物反应器生产口蹄疫新型口服疫苗奠定了基础。更多还原

【Abstract】 In this paper,structure protein gene P1 of foot and mouth disease virus was transformed into somatic embryos of soybean via A- grobacterium-mediated.The regenerated plantlets,which were resistant to kanamicy,were obtained and examined by PCR and PCR- southern blot.The results indicated that target gene had been transformed into soybean.It could afford the basic research for soybean as bioreactor to produce oral vaccines against FMDV.更多还原