【作者】 高海波； 陈孝； 陈明； 徐兆师； 李连城； 马有志；

【Author】 GAO Hai-bo~(1,2) CHEN Xiao~1 CHEN Ming~1 XU Zhao-shi~1 LI Lian-cheng~1 MA You-zhi~(1*) 1.Institute of Crop science,Chinese Academy of Agriculture Sciences,National Key Science Facility of Crop Gene Resources and Gene Improvement,Key laboratory of crop Genetics and Breeding,Ministry of Agricultrure,Beijing 100081,China; 2.College of Agronomy,Inner Mongolia Agricultural University,Mongolia Huhehot 010018,China

【机构】 中国农业科学院作物科学研究所农作物基因资源与基因改良国家重大科学工程,农业部作物遗传育种重点开放实验室；

【摘要】 以中间偃麦草基因组 DNA 为探针对小麦中间偃麦草异附加系 Z4进行基因组原位杂交分析,表明异附加系 Z4附加的一对中间偃麦草染色体与普通小麦的一对染色体发生了相互易位。对中间偃麦草、Z4和普通小麦品种宛7107进行 RAPD 分析, 在100条随机引物中,有两个引物 S1053和 S1068在 Z4中能扩出中间偃麦草的特异带。在利用 Z4进行小麦抗锈病育种时,可使用这两个标记进行辅助选择。更多还原

【Abstract】 The results of genomic in situ hybridization(GISH),in which Thinopyrum intermedium genome DNA was used as probe, proved that reciprocal translocation was accrued between a pair of Th.intermedium chromosomes and a pair of common wheat chromo- somes in Z4 additional line.RAPD analysis for Th.intermedium,Z4 and common wheat cv.Wan7107 showed that two primers-S1053 and S1068 amplified specific DNA bands of Th.intermedium in Z4 among the 100 random primers involved in this study.Both markers could be applied for assistant selecting when rust resistance breeding was carried out by using Z4 as resistant source.更多还原