【作者】 张丽； 张洪亮； 李自超；

【Author】 ZHANG Li ZHANG Hong-liang LI Zi-chao~* (Key Laboratory of Crop Genomics and Genetic Improvement of Ministry of Agriculture and Beijing Key Laboratory of Crop Genetic Improvement,China Agricultural University,Beijing 100094,China)

【机构】 中国农业大学农业部作物基因组学与遗传改良重点实验室北京市作物遗传改良实验室；

【摘要】 对同时含有 mtlD 和 BADH 基因的旱稻,只含 mtlD 基因或只含 BADH 基因的旱稻,非转基因旱稻三者在盐胁迫下的表型,生长速率,相对电导率以及 K+/Na+含量的比较,发现它们的耐盐性的关系是:同时含有 mtlD 和 BADH 基因旱稻＞只含 mtlD 基因或只含 BADH 旱稻＞非转基周旱稻,从而推断出 mtlD 基因和 BADH 基因在旱稻的耐盐性方面具有协同(或累加)效应。通过试验比较,还发现只含有 mtlD 基因和只含有 BADH 基因的旱稻的耐盐性没有明显差别,从而推断出 mtlD 基因和 BADH 基因对植物的耐盐性的贡献差异不显著。此外,已经得到的转基因材料也可以继续作为进一步转化的材料,这为我们进行多基因转化改良植物提供了一条新的途径。更多还原

【Abstract】 The salt-tolerant ability test including the phenotype,relative electric conductivity,and the K~+/Na~+ content indicates that the plants,which had been transformed two genes,had the highest salt-tolerant ability.The plant only with mtlD gene or only with BADH gene had secondly ability,and the nontransgenic plants was the third.So we concluded that the two genes(mtlD and BADH)have addi- tive effects of salt-tolerance.We also conclude that there is no difference between the effects of mtlD gene and BADH gene in salt-toler- ance.Besides,the transgenic material with one exogenous gene could be used as a new material for further transformation;this is a new road to improve a plant by polygenes transformation.更多还原