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不同生态环境下玉米产量性状QTL分析

QTL Analysis of Yield Components in Maize under Different Environments

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【作者】 兰进好李新海高树仁张世煌张宝石

【Author】 LAN Jin-Hao~(1,2);LI Xin-Hai~1;GAO Shu-Ren~1;ZHANG Shi-Huang~(1,①);ZHANG Bao-Shi~2 1. Institute of Crop Breeding and Cultivation, Chinese Academy of Agricultural Sciences/Key Laboratory of Crop Genetics and Breeding, Ministry of Agriculture, Beijing lO0081, China: 2. ShenyangAgriculture University, Shenyang 110161, China

【机构】 中国农业科学院作物育种栽培研究所/农业部作物遗传育种重点实验室沈阳农业大学

【摘要】 以玉米(Zea mays L.)自交系黄早四和Mo17为亲本得到的191个F2单株为作图群体,同时将衍生的184个F2:3家系作为性状评价群体,分析了单株穗数、穗行数、行粒数、百粒重和单株籽粒产量在北京和新疆两个生态环境下的表现,并比较了数量性状基因位点的定位结果。检测结果表明,在两个环境下共检测出47个QTL,分布于除第10染色体以外的9条染色体上,其中与单株穗数相关的QTL10个,可解释的表型变异为5.3%~25.6%;与穗行数相关的QTL13个,可解释的表型变异为4.5%~23.2%;与行粒数相关的QTL9个,解释的表型变异为5.4%~13.7%;与百粒重相关的QTL10个,可解释的表型变异为4.9%~13.3%;与单株籽粒产量相关的QTL有5个,可解释的表型变异为6.1%~35.8%。大部分产量QTL只在单一环境下被检测到,说明产量相关QTL与环境之间存在明显的互作。那些表型相关显著的产量性状,它们的QTL容易在相同或相邻标记区间检测到。研究发现了若干个QTL富集区域,可能是发掘通用QTL的侯选位点。

【Abstract】 One hundred and ninety-one F2 individuals derived from the cross, Mo17×Huangzao4, were genotyped by SSR and AFLP markers to construct the genetic linkage map, and 184 corresponding F2:3 families were phenotyped for maize (Zea mays L.) yield components in Beijing and Xinjiang. The performance and correlations among 5 yield components including ears per plant (EPP), row number per ear (RN), kernel number per row (KR), 100-kernel weight (KW) and grain yield per plant (GY) were evaluated, and the quantitative trait loci (QTL) were characterized. Totally, 47 QTLs were identified for 5 trails, locating on 9 chromosomes with exception of the chromosome 10.10, 13, 9, 10 and 5 QTLs were detected for EPP, RN, KR, KW and GY, respectively. Each of these QTLs could explain 5.3% to 25.6% phenotypic varialion of EPP, 4.5% to 23.2% of RN, 5.4% to 13.7% of KR, 4.9% to 13.3% of KW and 6.1% to 35.8% of GY. Most of the QTLs were detected in single environment, indicating the significant interactions between QTL and environments. Different QTLs relevant to the yield components which were correlated each other could be identified easily in the same or adjacent chromosome regions. Several regions with clustered QTLs relevant to multiple yield components identified in the study may provide the genetic loci of the universal yield QTLs.

【基金】 948重大国际合作研究(Q2003-3);国家863计划(2001AA211111);亚洲玉米生物技术协作网项目资助
  • 【会议录名称】 2004全国玉米种质扩增、改良、创新与分子育种学术会议论文集
  • 【会议名称】2004全国玉米种质扩增、改良、创新与分子育种学术会议
  • 【会议时间】2004
  • 【会议地点】中国北京
  • 【分类号】S513
  • 【主办单位】中国作物学会
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