【作者】 王易伟； 钟敏； 王安容；

【Author】 WANG Yi-wei.ZHONG Min, WANG An-rong. The Chongqing Plllmonology Hospital, Chongqing 400020

【机构】 重庆市肺科医院；

【摘要】 目的探讨依赖利福平结核分支杆菌(依R菌)在BACTEC系统中的生长特征,以便用该系统鉴定依R菌。方法选取L-J法初步鉴定的依R菌,制成菌液(10-4mg/ml)分别以0.1ml接种于含不同利福平(R)浓度(1～384μg/ml)的7H-12液体培养基中培养,用BACTEC法测定不同实验瓶生长指数(GI),判断耐药性,并对培养物称重及进行形态(抗酸染色)观察。结果(1)耐药性,依R菌在R 1～96μg/ml 10个浓度中均耐药,R 192 μg/ml临界,384μg/ml敏感。(2)菌体重量,R 6、8、10、12、24μg/ml各瓶细菌重量(分别为87、125、116、104、65mg)明显高于对照瓶(35mg),重量比为1.9-3.6,平均2.9,其余各耐药瓶与对照瓶无明显差别。(3)培养物涂片抗酸染色,可见各R瓶内结核分支杆菌菌体比对照瓶粗大、染色质深、染色颗粒明显,以R 8μg/ml瓶最为典型。结论依R菌在含R(6-24μg/ml)环境中生长旺盛,在无R环境中生长不良。更多还原

【Abstract】 Objective To analyze the growth and drug resistant characteristics of rifampin-depending Mycobacterium tuberculosis strains(rdm) in BACTEC in order to identify rdm in clinic. Methods The rdm was indemnified by the previous L-J cultivation.Suspension(10-4 mg/m10.1ml) was inoculated in 7H12 liquid media containing lifampin. Their growth index(GI) was detected by BACTEC and the bacteria in the media were weighted and stained by acid-fast siaining. Results The resistance to rifampin was detected in media containing 1-96μg/ml of rifampin.Thesensitivity to rifampin was detected in the media containing 384μg/ml of rifampin.The weight of the rdm in the media containing6,8,10,12 and 24μg/ml was87,125,116,104 and 65mg respectively.that were marked higher than that of control (35mg).The weight of rem in the media containing rifampin(1 92-384μg/ml) was 1.9-3.6 times that of control .The bodies of rdm in the media containing rifampin were larger and deeper stained in the acid-fast staining compared with the control.Conclusion The rdm grows vigorously in media containing rifampin of 6-24 μg/ml,but it grows much weakly in the media containing no rifampin.更多还原