【作者】 姜国华； 黄建华； 刘忠敏；

【Author】 JIANG Guo-hua, HUANG Jian-hua, LIU Zhong-min (Centre of Analytical and Testing, Beijing Normal University, Beijing 100875, China)

【机构】 北京师范大学分析测试中心；

【摘要】 本研究涉及应用Idogn法对神经生长因子(NGF)进行1251标记,并用同位素示踪法与电泳法相结合的方法研究125I-NGF在小鼠体内的血药浓度-时间过程。结果显示,静脉注射125I-NGF在小鼠体内的代谢规律符合二房室开放模型。分布相半衰期为0．13 h,消除相半衰期为3．68 h,125I-NGF在小鼠体内分布和消除均较快,清除率为0．125 L·h-1·kg-1,表观分布容积为0．697 L·kg-1,曲线所围面积为 16．01μg·h·L-1。更多还原

【Abstract】 The study concerns in the label of nerve growth factor(NGF) by 125I with Idogn method. Course of time - 125I-NGF concentration in plasma of mouse was investigated with the method of sodium dodecylsulfate-polyaccrylamide gel electrophoresis (SDS-PAGE) after iv 125I-NGF. Result demonstrates the curve of time- 125I-NGF concentration in plasma after iv fit a two compartment open model. Distribution phase half life is 0. 13 h, elimination phase half life is 3. 68 h, the mean plasma clearance is 0. 125 L · h-1 · kg-1 , apparent volume of distribution is 0. 697 L · kg-1 , and the area under curve is 16. 01 g · h · L-1.更多还原