【作者】 李亚明； 张晶； 赵军； 陈煜； 段钟平；

【Author】 LI Ya-ming ZHANG Jing ZHAO Jun CHEN Yu DUAN Zhong-ping. Artificial Liver Treatment and Training Center,Beijing Youan Hospital,Capital University of Medical Sciences,Beijing 100054,China

【机构】 首都医科大学附属北京佑安医院人工肝中心；

【摘要】 目的:建立大动物急性肝功能衰竭动物模型,用来评价非生物型组合人工肝治疗效果。方法: 将中国实验小型猪28头随机分为对照组(n=13)与治疗组(n=15),应用 D-氨基半乳糖1.2g/Kg 静脉注射诱导建立急性肝衰竭动物模型。给药48小时后治疗组给予血浆置换联合血液滤过治疗,对照组未行任何干预措施。观察比较两组动物生存时间、生化指标、颅内压与病理变化。结果:治疗组的生存时间较对照组明显延长,两组分别为128.7±11.3小时和67.9±9.4小时(P<0.01),治疗组多项生化指标、炎性细胞因子、颅内压、组织病理等都比同期非治疗组动物有不同程度改善(P<0.05～0.01)。结论:血浆置换联合血液滤过的非生物型组合人工肝治疗,能显著延长急性肝衰实验动物生存时间,降低颅内压,改善生化、病理指标与近期预后,可作为肝脏移植和药物治疗的有效辅助手段。更多还原

【Abstract】 Objective:To evaluate the efficacy of combined nonbioartificial liver(plasma exchange plus continuous hemofiltration)for large animal model of fulminant hepatic failure induced by D-galactosamine. Methods:28 pigs were divided into two groups at random:control group(n=13)and treatment group(n=15). All of the animals were induced fulminant hepatic failure with administration of D-galactosamine firstly.48 hours later the treatment group was treated with PE combined with CHF for 12 hours.Efficacy was assessed by comparing the survival time,improvement in manifestation,biochemical and pathological parameters.Results: The treatment group’s mean survival time was128.7±11.3 hours,which was much longer than in the control group67.9±9.4 hours(p<0.01).There were significant differences for serum bilirubin,aminotransferase,ICE ammonia,prothrombin time and histological manifestation between the two groups at the same time. Conclusion:Combined nonbioartificial liver(PE plus CHF)was effective for fulminant hepatic failure,and may be as a good bridging measure for liver transplantation and other method.更多还原