【作者】 阎明； 朱孔锡； 孟繁立； 王红娟； 吴美玲；

【Author】 Yan Ming Zhu Kong-xi Meng Fan-li,Department of gastroenterology,Qi Lu hospital,Shah Dong University,Ji nan 250012,China

【机构】 山东大学齐鲁医院消化科；

【摘要】 目的观察乙醛脱氢酶(ALDH)基因多态性与山东地区汉族人群酒精性肝病的关系,探讨酒精性肝病遗传学的发病机制。方法采用 PCR 结合内切酶酶切及电泳技术,检测山东地区汉族人群中酒精性肝病组、嗜酒组和对照组(各20例)中 ALDH 各基因型及等位基因的频率并进行比较。结果 ALDH2~*1、ALDH2~*2两种等位基因在对照组与酒精性肝病组之间的分布差异有显著性(x~2=4.80, p<0.05),对照组与无肝病嗜酒组之间无明显差异(P>0.05),在嗜酒组和酒精性肝病组中以 ALDH2~*1/~*1 基因型为主,均未检出纯合子的 ALDH2~*2/~*2。结论 ALDH 基因多态性与酒精性肝病的发生关系密切。等位基因 ALDH2~*2可能是本地区汉族人群中嗜酒和酒精性肝病的负性危险因素。更多还原

【Abstract】 Objective:By detecting the relationship between ALDH gene polymorphism and alcoholic liver disease,to investigate the genetic pathogenesis of ALD.Methods:By using PCR and restriction endonuclease and electrophoresis methods,to detect the genotypes and alleli of ALDH2 gene in the healthy control group,alcohol dependent group and alcoholic liver disease (ALD) group,each group contains 20 patients.Results:The frequency of ALDH2* 1 and ALDH2*2 alleli has statistic significance between healthy control group and ALD group (x~2=4.80,p<0.05),and has no statistic significance between healthy control group and alcohol dependent group (P>0.05).Conclusion:.The gene polymorphism of ALDH has statistic relationships to ALD,it may play an important role in the pathogenesis of ALD,the allele of ALDH2*2 may be a negative risk factor for the developing of ALD.更多还原