【作者】 曹春雨； 杜贵友； 左萍萍； 刘显峰； 王文韬； 赵雍； 王秀荣； 崔海峰；

【Author】 CAO Chun-yu,DU Gui-you,ZUO Ping-ping~*,LIU Xian-feng~(**), WANG Wen-tao~(**),ZHAO Yong,WANG Xiu-rong,CUI Hai-feng (*) Department of Pharmacology,Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100005,China; * *) Wan Xi Pharmaceutical Factory,Xixia 474550,Henan,China)

【机构】 中国医学科学院中国协和医科大学基础医学研究所； 河南宛西制药厂；

【摘要】 目的:探讨天麻促智颗粒(TMC)的脑保护机制,为其治疗老年血管性痴呆的作用提供药理学依据。方法:利用小鼠双侧颈总动脉结扎-再灌手术造成的学习记忆障碍动物模型,观察不同剂量的TMC对脑内神经递质受体(N-甲基-D-天冬氨酸受体即NMDA受体、胆碱能M受体)及谷胱甘肽过氧化物酶(GPx)活性的影响。结果:TMC可不同程度地抑制小鼠脑损伤引起的大脑皮层、海马两部位NMDA受体活性;增强两部位的M受体活性;还可增加皮层、海马和肝脏中GPx的活性。结论:TMC治疗血管性痴呆的机制可能与其抑制脑内兴奋性氨基酸受体活性、提高胆碱能神经功能以及增加抗过氧化酶活性有关。更多还原

【Abstract】 Objective:To provide pharmacological evidence for the protective effects of Tianmacuzhi Granules(TMC) in mouse brain in a ischemia-reperfusion model.Method:Receptor binding test of NMDA receptor and M receptor in brain,GPx activity determination in brain and liver effected by different dose of TMC were performed.Result:TMC could reduce the NMDA receptor activitiy,increase the M receptor activity and GPx activity of cerebral cortex and hippocampus tissues significantly.Conclusion:TMC might antagonize ischemia-reperfusion injury of brain by reducing the glutamate neurotoxicity and increasing the function of cholinergic nerve and effects of anti-peroxidization in brain.更多还原