【作者】 许明淑； 黄璐琦； 付梅红； 叶和春； 李国凤；

【Author】 XU Ming-shu,HUANG Lu-qi,FU Mei-hong,TE He-chun~*,LI Gou-fen~* (* Institute of Botany,Chinese Academy of Sciences,Beijing 100093)

【机构】 中国科学院植物研究所；

【摘要】 目的:诱导出贯叶金丝桃的愈伤组织,并确定其中含有金丝桃素。方法:在不同条件下采用组织培养技术,进行贯叶金丝桃的愈伤组织诱导研究,用HPLC方法确定其中含有的金丝桃素。结果和结论:最佳诱导条件是MS基本培养基加入生长调节物质2,4-D(4μg·L^-1)和6-BA(0.2μg·L^-1)。叶腋等分生组织较多的部位易于诱导愈伤组织。诱导出的愈伤组织,经HPLC方法确定其含金丝桃素。更多还原

【Abstract】 Objective:To induce the callus of H.perforatum and identify hypericin and pseudohypericin of its callus.Method:The callus was induced in different culture conditions, and active constituents were determined by HPLC.Result and conclusion:The inductions of callus from different parts were discussed,the induction rate of the leaf axil being the highest.The MS basic medium with 4μg·L^-1 2,4-D and 0.2μg·L^-1 6-BA was the best of all screened media.Hypericin in the callus is determined by HPLC.更多还原