海洋放线菌N350菌株产生的蒽环类抗肿瘤活性物质

【作者】 苏文金； 郑忠辉； 黄耀坚； 李军； 曾伟； 蔡慧农；

【机构】 厦门大学生物学系； 集美大学水产生物技术研究所；

【摘要】 对海洋放线菌N350菌株产生的抗肿瘤活性物质进行早期鉴别,结果表明该活性物质具有酸碱指示性质,化学显色结果和阿霉素相似;其中性甲醇溶液在λ233nm、252nm、480nm、494nm等处有特征吸收峰,加入0.5%醋酸镁甲醇溶液后,溶液颜色变紫,可见吸收峰在540nm和579nm处OD值增大,在0.1NNaOH甲醇溶液中峰型红移,且E556nm/E596nm=1.11;其盐酸水解物的HPLC检测结果显示与柔红霉酮有一相同的出峰时间。提示N350产生的抗肿瘤活性物质属于蒽环类柔红霉素族。更多还原

【Abstract】 The purpose of this paper was to analyse the antitumor agent produced by a marine actinomycetes strain N350.The results showed that this antitumor agent had the property of pH indicator and identical chemical color development results with those of anthracyclines,such as adriamycin.The other physico-chemical properties of this antitumor agent,including specific absorptance peaks atλ233nm,252nm,480nm,494nm in neutral MeOH solution,the absorptance atλ540nm andλ579nm turning to be higher when solved in 0.5% Mg（OAC）₂-MeOH while the color of the solution becoming purple,the ratio of E556nm/E596nm being 1.11 in 0.1N NaOH-MeOH and its aglycone having the same retention time of HPLC as daunomycinone,demonstrated that it belongs to the same group of daunomycin.更多还原