【作者】 王吉成； 钱蓓丽；

【Author】 WANG Ji-Cheng,QIAN Bei-Li (Shanghai Institute of Pharmaceutical Industry,Shanghai 200437)

【机构】 上海医药工业研究院；

【摘要】 <正>单细胞微量凝胶碱性电泳试验(alkaline single cell micro gel electrophoresis assay,SCGE),又名慧星试验(comet assay),可以在单个细胞水平检测受试物诱发的哺乳动物乃至人体细胞DNA单链断裂(single strand break,SSB)、遇碱不稳定位点(alkaline labile sites,ALS)等多更多还原

【Abstract】 The single cell microgel electrophoresis assay（comet assay） can detect DNA single strand breaks,alkaline labile sites and other genetic damages.Compared with traditional DNA break test methods,comet assay is more quick,sensitive,convenient and visual.Particularly,it is applicable to various mammalian cell types,needs much less cells,and does not need radio-active isotope labeling.It can study the genotoxic specificity at the single cell level,therefore can be used to screen drug genotoxity.In this study,the genotoxicities of 34 chemicals to mouse lymphocytes were further tested by in vitro comet assay.The test substance included known genotoxicants, antineoplastic drugs,and flavone derivatives.Among the test chemicals,the concordance of present study with Ames test was 62.1%（18/29）,and the comparison of present results with animal carcinogenecity test data showed that the sensitivity was 75.0%（15/20）,and the concordance,73.9 （17/23）,respectively.Flavone derivatives SIPI-644（10μM） and SIPI-1211（33μM ） were positive in the presence of S₉ while others were negative.Therefore the comet assay can be used to detect genotoxicants of various mechanisms including free radicals,alkylating of nucleotide,DNA adduct, strand break,etc.This indicated that further in vivo model could be explored to screen drug genotoxicity when combined with the general toxicity test and the pharmacokinetic study.更多还原