姜黄素对前列腺癌DU-145细胞TGF-β/Smads信号传导通路的影响

【作者】 张蜀武； 王林； 周士轶； 丁维俊；

【机构】 成都中医药大学附属医院； 四川邛崃市医疗中心医院男科； 成都中医药大学临床医学院； 成都中医药大学基础医学院；

【摘要】 目的:探讨中药提取物姜黄素对人雄激素非依赖性前列腺癌DU-145细胞TGF-β/Smads信号传导通路的影响,从而探讨姜黄素抑制DU-145细胞的机理。方法:分别用12.5μmol/l、6.25μmol/l以及3.125μmol/l姜黄素作用DU-145细胞48h后,以光镜观察DU-145细胞形态变化,以MTT法检测DU-145细胞生长情况,以RT-PCR法检测TGF-β,SMAD2,SMAD4和SMAD7基因表达情况。结果:姜黄素能显著抑制DU-145细胞生长,呈一定的量效关系;RT-PCR法检测DU-145细胞TGF-β,SMAD2和SMAD4基因表达均不同程度增加,而SMAD7的基因表达不同程度减少。结论:姜黄素通过对TGF-β及下游蛋白Smad2、Smad4、Smad7的影响,进而激活或调节TGF-β/Smads信号传导通路,这可能是姜黄素促进DU-145细胞凋亡和抑制其生长的重要分子机制。更多还原

【Abstract】 Objectives:To observe the efficacy Curcumine having on TGF-β/Smads pathway of human DU-145 prostatic cancer cells in hormone-independent prostatic cancer,and to accordingly observe the principle of it restraining DU-145 cells.Methods:Using 12.5μmol/l,6.25μmol/l and 3.125μmol/l Curcumine concentrations on DU-145 cells for 48 hours each.The DU-145 cells’ morphology was observed under optic microscope.The multiplication of DU-145 cells was measured by MTT method.RT-PCR methods was used to measure the expressions of TGF-β,SMAD2,SMAD4 and SMAD7.Results:The growth of DU-145 cells was suppressed obviously by curcumine and a certain quantity-effect relation was shown in each concentration group.The expressions of TGF-β,SMAD2 and SMAD4 were increased by the concentration groups measured with RT-PCR but SMAD7 were lowed.Conclusions:To intervention TGF-β,SMAD2,SMAD4 and SMAD7 of human DU-145 prostatic cancer cells,curcumine can activation or regulate TGF-β/Smads pathway of them,what could be the important mechanism of curcumine restraining DU-145 cells.更多还原