【作者】 邢秀梅； 杨福合； 张兆刚； 苏伟林； 李一清；

【Author】 XING Xiu-mei,YANG Fu-he,SU Wei-lin,LI yi-qing (Institute of Wild Plant and Animal Science CAAS,Jilin 132109)

【机构】 中国农业科学院特产研究所吉林省特种经济动物分子生物学省部共建实验室特种经济动物种质资源遗传改良重点开放实验室； 黑龙江省宁安农场鹿业公司；

【摘要】 采用聚合酶链式反应(PCR)技术对40个马鹿mtDNA细胞色素b序列进行扩增,所得PCR产物测序,测得405bp的核甘酸片段序列,统计分析了6个品种或类型间的遗传关系,通过遗传关系建立了系统发育树。结果表明天山马鹿和东北马鹿的亲缘关系较近,塔里木马鹿同天山马鹿、阿尔泰马鹿、甘肃马鹿、东北马鹿、左家马鹿的亲缘关系较远。塔里木马鹿和天山马鹿的亲缘关系最远。将原来6个马鹿品种(或类型)分为4大类群,东北马鹿为一类,天山马鹿和阿尔泰马鹿为一类、塔里木马鹿为一类、甘肃马鹿为一类。更多还原

【Abstract】 The PCR technique was used to amplify the mtDNA cyt b in 40 individuals of wapiti.The PCR products were purified and cloning sequencing was conducted.405bp nucleotide sequences of partial were obtained.Statisticsed to analyze 6 specieses or the heredity of the type a relation,pass heredity a relation to build up system growth a tree.It shows that the Tianshan wapiti has a close relation to the northeast Wapiti,but the Talimu Wapiti has a for relationship between the Tianshan Wapiti、the Aertai Wapiti、the Gansu Wapiti、the northeast Wapiti and the Zuojia Wapiti,furthermore the Talimu Wapiti has the farthest relationship with Tianshan Wapiti.The results of the study is that. Six species(or type) are divided into 4 groups,that is the group of the northeast Wapiti,of Tianshan and Aertai Wapiti,of Talimu Wapiti and of Gansu Wapiti.更多还原