【作者】 曾显营； 田国彬； 冯华朋； 邓国华； 施建忠； 张盼涛； 苏文； 杨婧； 刘景利； 徐佳； 陈化兰；

【Author】 ZENG Xianying 1,TIAN Guobin (1),FENG Huapeng 1,DENG Guohua 1,SHI Jianzhong 1, ZHANG Pantao 1,SU Wen 1,YANG Jing 1,LIU Jingli 2,XU Jia 2,CHEN Hualan (1) (1.State Key Lab of Veterinary Biotechnology /Animal Influenza Laboratory of the Ministry of Agriculture,Harbin Veterinary Research Institute,CAAS,Harbin 150001; 2.Harbin Weike Biotechnology Development Company,Harbin 150001)

【机构】 中国农业科学院哈尔滨兽医研究所,兽医生物技术国家重点实验室,农业部动物流感重点开放实验室,国家禽流感参考实验室； 哈尔滨维科生物技术开发公司；

【摘要】 本研究对经反向遗传方法构建的重组禽流感H5N1亚型变灭活疫苗种毒Re—6株的生物学特性及免疫效力进行研究。将Re—6株接种SPF鸡胚后37℃培养72h,鸡胚存活,且无病变,HA滴度达2¹⁰;以0.1ml(10^6.0EID₅₀/0.1ml)的剂量鼻腔感染4周龄SPF鸡10天后血清HI抗体转阳,无任何症状,也不排毒;SPF鸡静脉致病指数（IVPI）为0;以Re—6重组株为种毒制备灭活疫苗,免疫SPF鸡和SPF鸭后,3周时平均HI抗体效价达8.3log2和8.4 log²;免疫鸡和免疫鸭对亲本强毒株GD1322攻击提供完全保护。以上结果表明禽流感灭活疫苗种毒Re-6株对SPF鸡胚和SPF鸡无致病性、适合鸡胚增殖、抗原针对性强,且该毒株制备的灭活疫苗具有良好的免疫效力,是理想的疫苗种毒株。更多还原

【Abstract】 An inactivated vaccine seed strain（Re-6）has been generated by reverse genetics from the avian influenza virus（GSl322）.In this study,we evaluated the biological characteristics and immunogenicity of Re-6 strain.When SPF embroynated chicken eggs were inoculated with Re-6 strain and incubated at 37℃for 72 hours,all the eggs were alive and the HA titer in eggs could reach to 2¹⁰.No virus shedding could be detected form either trachea or cloaca swabs after 4-week SPF chickens were inoculated intranasally with 10^6.0 EID50 of Re-6 strain,and all the chicken had seroconverted by day 10 p.i..IVPI value of Re-6 was 0.An inactivated vaccine derived from Re-6 strain could induce high HI antibody（8.3log2 and 8.4log2,respectively）in SPF chickens and ducks 3 weeks post-immunization（P.V.）;the immunized chickens and ducks could be completely protected from the homologous virus GD1322.The nonpathogenic,high growth and good immune efficacy made Re-6 strain an ideal seed for vaccine production to prevent the spread of the clade 2.3.2.1 H5N1 avian influenza virus variant.更多还原