【作者】 单芬； 郑煦灿； 韦良孟； 徐成刚； 罗开健； 任涛； 辛朝安； 焦培荣； 廖明；

【Author】 SHAN Fen~(1,2),ZHENG Xucan~1,WEI Liangmeng~1,XU Chenggang,LUO Kaijian~1, REN Tao~1,XIN Chaoan~1,JIAO Peirong~(1*),LIAO Ming~(1*) (1.Key Lab of Animal Disease Control and Prevention of Ministry of Agriculture,South China Agricultural University,Guangzhou,Guangdong 510642;2.Guangdong Winsun Biopharmaceutical Limited Company, Guangzhou,Guangdong 511356)

【机构】 华南农业大学兽医学院农业部动物疫病防控重点开放实验室； 广东永顺生物制药有限公司；

【摘要】 2005年在广东进行流行病学调查时分离到一株鹦鹉源禽流感病毒,经鉴定为H5N2亚型禽流感病毒(A/Parrot/Guangdong/268/2005)。该毒株的HA裂解位点附近的氨基酸序列为RETRGLF,只含有一个碱性氨基酸,符合低致病性禽流感病毒的HA裂解位点附近氨基酸序列的分子特征;与H5N2亚型禽流感代表毒株相比,该毒株HA和NA基因的糖基化位点、HA基因的受体结合位点编码区、NA基因的耐药性位点均未发生变异。将该毒株全基因组序列与GenBank已公布的19株H5N2亚型禽流感病毒株的相应序列进行比较分析并绘制系统进化树后发现:其与低致病性禽流感毒株A/Pheasant/NJ/1355/1998(H5N2)-like的亲缘关系最近,位于以A/Chicken/Pennsylvania/1/1983(H5N2)为代表的美洲进化分支。更多还原

【Abstract】 In 2005,an avian influenza virus stain was isolated from Parrot in Guangdong,which was then genotyped as H5N2 subtype and designated as A/Parrot/Guangdong/268/2005.According to the current OIE definition on the low-pathogenicity of avian influenza virus,the strain was recognized as a low pathogenic avian influenza virus due to the present of one basic amino acid residue at the HA cleavage site. Some molecular characteristics of the virus,such as potential glycosylation sites in HA and NA,receptor binding sites of HA,drug resistance site of NA and so on,have no variations.To analyze molecular evolution of this strain,we selected the sequences of H5N2 subtype AIVs from GenBank and established the phylogenetic trees for them.Our results indicated that this strain shared the highest homologies with the H5N2 LPAI isolate A/Pheasant/NJ/1355/1998-1ike.Phylogenic analysis revealed the isolate,together with A/Chicken/Pennsylvania/1/1983(H5N2) was mapped to America lineages and clustered with A/Pheasant/N J/1355/1998-1ike.更多还原