【作者】 王彦妮； 曾显营； 田国彬； 苏文； 陈化兰；

【Author】 WANG Yan-ni ZENG Xian-ying TIAN Guo-bin~* SU Wen CHEN Hua-lan~* (Animal Influenza Key Laboratory of the Ministry of Agriculture,State Key Laboratory of veterinary Biotechnology,Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences,Harbin 150001)

【机构】 中国农业科学院哈尔滨兽医研究所农业部动物流感重点开放实验室/兽医生物国家重点开放实验室；

【摘要】 为了解肉鸡生长期内免疫前后的细胞免疫反应,本试验采用CD3/CD4/CD8三色流式细胞术、MTT法和Griess法依次检测了AA肉鸡免疫禽流感灭活疫苗和不免疫状态下外周血、胸腺、脾脏中T淋巴细胞及其亚群的相对含量、外周血中T淋巴细胞对PHA-P的增殖反应能力和诱导巨噬细胞NO分泌量的变化。结果表明:不免疫状态下,AA肉鸡外周血CD4+、CD8+、CD3+T淋巴细胞相对百分含量在7日龄左右时均最低,脾脏中CD4+T淋巴细胞相对百分含量在整个试验期一直处于下降状态,免疫后外周血、脾脏和胸腺中CD4+、CD8+、CD3+T淋巴细胞相对百分含量均明显高于未免疫组,且脾脏CD4+T淋巴细胞呈阶段性上升状态;各时期SI值和巨噬细胞NO分泌量检测结果表明,免疫组均高于同期对照组(p<0.05)。研究结果还表明:肉鸡在7日龄时体液免疫和细胞免疫水平最低,灭活疫苗也可以激发肉鸡产生较强的细胞免疫反应。更多还原

【Abstract】 To understand cellular immune response of immunized AA broiler during the growing period, we detected the relative amount of T lymphocyte and its subgroups isolated from periphery blood,thymus and spleen,the ability of proliferative response of T lymphocyte to PHA-P and the changes of NO secretory volume from induced rnacrophages from periphery blood between immunized chickens and unvaccinated ones through CD3/CD4/CD8 triple labelling flow cytometricanalysis,MTT assays and Griess assays, respectively.The result indicated that the relative percent of CD4+ and CD8+ subsets,CD3+ cells T lymphocytes in periphery blood kept the lowest level on 7-day old,and the relative percent of CD4+ T lymphocytes in spleen constantly decreased during the process of whole research in the unvaccinated AA broiler.While the relative percent of CD4+ and CD8+ subsets,CD3+ cells T lymphocytes in periphery blood, spleen and thymus of immunized groups was higher than the unvaccinated groups,and the relative percent of CD4+ T lymphocytes in spleen consistently increased after vaccination.The detection result of SI-value and NO secretory volume of macrophages in the different stage displayed that immunized groups higher then control of the same experimental period(p<0.05).The whole research also suggested that humoral immunity and cellular immunity stayed the lowest level in the first week and inactivated vaccine can also stimulate strong cellular immune response.更多还原