【作者】 马鸣超； 姜昕； 刘菲； 李俊；

【Author】 MA Ming-Chao~1,JIANG Xin~(1,2),LIU Fei~1,LI Jun~2.(1.School of Water Resources and Environment, China University of Geosciences/ Beijing Key Laboratory of Water Resources & Environmental Engineering,Beijing 100083,China;2.Institute of Agricultural Resources and Regional Planning,Chinese Academy of Agriculture Science, Beijing 100081,China)

【机构】 中国地质大学(北京)水资源与环境学院/中国地质大学(北京)水资源与环境工程北京市重点实验室； 中国农业科学院农业资源与农业区划研究所；

【摘要】 为了研究污水人工快速渗滤系统（CRI）中氨氧化菌的空间分布规律以及NH₄-N在CRI中的迁移转化机理,从深圳运行中的CRI系统砂层填料中以5cm～10cm间隔垂直取12个样品,对其氨氧化菌的16SrDNA进行DGGE分析,结果表明,CRI系统中氨氧化菌菌群随着深度的增加先增多后减少,在表层至10cm处氨氧化菌约有4、5种组成,主要由某些以有机氮为降解底物的微生物和进水中被砂粒介质截流吸附的微生物组成;在20cm～90cm范围内氨氧化菌增加至9种左右,多样性最为丰富,是亚硝化作用发生的主要区域;而在100cm以下的范围,由于NH₄-N浓度的降低或系统复氧不足,氨氧化菌迅速减少至5、6种。本研究结果证明了氨氧化菌在快渗池内分布范围的变化对NH₄-N去除率的影响,即氨氧化菌在快渗池内分布的越接近中上层,对NH₄-N的去除效果越好。本文关于CRI系统中氨氧化菌的空间分布规律及其与NH₄-N去除率关系的认识,为工艺改进和提高效果提供了理论基础。更多还原

【Abstract】 In order to reveal the microbial community distribution of ammonium oxidizing bacteria and the removal mechanism of NH₄-N in Constructed Rapid Infiltrition system（CRI）,sands of different depth were sampled from CRI in ShenZhen,and were analyzed by Denaturing Gradient Gel Electrophoresis（DGGE ）.The profiles of DGGE show that the microbial community of ammonium oxidizing bacteria increases followed by a decrease from top to bottom.There are about 4-5 species in the upper 10cm part,and they are composed of microorganism in sewage or those which played important roles in the biodegradation of organonitrogen.About 9 species are found in CRI between 20cm and 90cm in depth,where the nitrosation capacity is stronger.For lack of NH₄ -N or oxygen,the species of ammonium oxidizing bacteria reduce to 5-6 below 100 cm part.The results show that,the upper the ammonium oxidizing bacteria distribute in CRI,the higher the removal rate of NH₄-N will be.The relationship between community distribution of ammonium oxidizing bacteria and the removal of NH₄-N is meaningful to improve the treatment performance of CRI.更多还原