【作者】 李荣森； 戴顺英； 高梅影； 彭可凡；

【Author】 LI Rongsen DAI Shunying GAO Meiying PENG Kefan (Wuhan Institute df Virology,Chinese Academy of Sciences,Wuhan 430071,China)

【机构】 中国科学院武汉病毒研究所；

【摘要】 <正>1989年自云南昆明市石林的红棕壤中分离到数株苏云金杆菌(Bacillus thuringiensis)菌株,对其中的一株YK30-04进行了生物学特性、杀虫活性研究及分类鉴定。该菌株为周生鞭毛,产生双锥形伴孢晶体。晶体蛋白质由135kDa和130 kDa两种蛋白质组成。该菌株对小菜蛾(Plutella xylostella)和斜纹夜盗蛾(Prodenia litura)幼虫具有低毒力,对黄粉甲(Tenabrio molitor)和致乏库蚊(Culax fatigans)无毒效。该菌株的抗血清与H1-H69标准菌株H抗更多还原

【Abstract】 A new strain YK30-04 of Bacillus thuringiensis was isolated from soil in Yunnan Province,Kunming in 1989.It was determined as a new serotype H70 by agglutination tests.Parasporal crystals were bipyramidal shape and contained two proteins of 135kD and 130kD.Strain YK30-04 showed lower toxicities to the larva of Plutella xylostella and Prodenia litura,whereas no activities to the larva of Tenebrio molitor and Culex fatigans.Thus the strain YK30-04 was identified as a new serovar:Bacillus thuringiensis subsp.sinensis.更多还原