【作者】 缪文超； 李廷宝； 陈文辉； 朱金国； 吕红；

【Author】 Miao Wenchao Li Tingbao Chen Wenhui Zhu Jinguo L(?) Hong

【机构】 湖南省微生物研究所；

【摘要】 <正>自本世纪30年代以来白僵菌制剂的生产,主要采用固体发酵法,使其产生分生孢子。前苏联曾进行过液体深层发酵,但未见大规模工业生产的报道。在我国多采用曲盘等固体发酵生产,也有采用液体浅层培养白僵菌收集分生孢子和液体更多还原

【Abstract】 A novel technology for commercial production of B.Bassiana is reported in the present paper.Medium in a 1500 L fermenter was inoculated with culture of a 30 L seed fermenter and incubated for 24-36 hr and then transferred to a loose carrier in nylon screen trays,with an inoculum-medium ratio of 1:3.5（v/v）.After growing for 5 days,sporulation finished when culture thickness was 3-6cm at 25℃.The culture was dried at 35℃and then conidia were collected with a collector of Type 24ZC-1-1 at a rate of 9.45 kg/hr,yielding a conidial powders of 10¹¹ conidia/g. The collecting rate of powders to culture was about 1/10（v/v）,with a conidial recovery at 88%.The germination rate of conidia was about 92%.The cost of the product was 33900 yuan RMB per ton.更多还原