节点文献
球孢白僵菌对马尾松毛虫的毒力与有关生化指标的关系 Ⅱ.不同世代、不同菌株过氧化物酶、脂肪酸与毒力关系
RELATION OF VIRULENCE OF BEAUVERIA BASSI ANA AGAINST DENDROLIMUS PUNCTATU S TO SOME BI OCHEMICAL ESTIMATES Ⅱ.PEROXIDASE ISOZYME AND FATTY ACIDS OF DIFFERENT STRAINS AND DIFFERENT SUBCULTURES OF BEAU AERI A BASSI AN A AND RELATION TO THEIR VIRULENCE
【Author】 Fan Meizhen Peng Huanming Shen Kei (Northwest College of Forestry,Yangling,Shanxi) Li Hua (Forestry Department of Suburban District,Yingchuan,Ningxia)
【摘要】 <正>材料与方法(一)过氧化物酶的测定1.菌种:不同世代及不同菌株白僵菌同Ⅰ,培养同Ⅰ。2.过氧化物酶的测定:刮取供试菌丝2g,加入3ml Tris-Hcl缓冲液(pH=8.0)及适量石英砂,冰浴研磨匀浆,离心30分钟(4000rpm/min),取上清液贮于4℃冰箱备用。同
【Abstract】 In the present study,peroxidase isozyme and fatty acids of 29 strains and different subcultures(1st and 5,10,15,20,25,30,35,and 40th) of 2 strains of Beauveria bassiana were studied and the results were compared to those of a bioassay.The isozyme study result revealed very limited peroxidase bands(2 bands at most).The zymograms varied irregularly and showed no association to virulence.The fatty acid analysis showed that the fatty acid composition of the subcultures from one strain was essentially the same,but the contents of all fatty acids varied irregularly and showed no correlation to the change of their virulence.The fatty acid composition of 7 strains were essentially the same and the change of any fatty acid was not correlated to the decline of the virulence of the strains.
- 【会议录名称】 中国虫生真菌研究与应用
- 【会议名称】第三届全国虫生真菌学术讨论会
- 【会议时间】1991-10-16
- 【会议地点】中国福建福州
- 【分类号】S763.306.4
- 【主办单位】中国菌物学会虫生真菌专业委员会