【作者】 樊美珍； 逄焕明； 沈奎； 李华；

【Author】 Fan Meizhen Peng Huanming Shen Kei (Northwest College of Forestry,Yangling,Shanxi) Li Hua (Forestry Department of Suburban District,Yingchuan,Ningxia)

【机构】 西北林学院； 银川市郊区林业局；

【摘要】 <正>材料与方法(一)过氧化物酶的测定1.菌种:不同世代及不同菌株白僵菌同Ⅰ,培养同Ⅰ。2.过氧化物酶的测定:刮取供试菌丝2g,加入3ml Tris-Hcl缓冲液(pH=8.0)及适量石英砂,冰浴研磨匀浆,离心30分钟(4000rpm/min),取上清液贮于4℃冰箱备用。同更多还原

【Abstract】 In the present study,peroxidase isozyme and fatty acids of 29 strains and different subcultures(1st and 5,10,15,20,25,30,35,and 40th) of 2 strains of Beauveria bassiana were studied and the results were compared to those of a bioassay.The isozyme study result revealed very limited peroxidase bands(2 bands at most).The zymograms varied irregularly and showed no association to virulence.The fatty acid analysis showed that the fatty acid composition of the subcultures from one strain was essentially the same,but the contents of all fatty acids varied irregularly and showed no correlation to the change of their virulence.The fatty acid composition of 7 strains were essentially the same and the change of any fatty acid was not correlated to the decline of the virulence of the strains.更多还原