【作者】 张青文； 王秋旗； 罗科； 周友泉；

【Author】 Zhang Qingwen Wang Qiuqi Luo Ke Zhou Youquan (Bingjing Agricultural University,Beijing 100094)

【机构】 北京农业大学；

【摘要】 深绿拟青霉(Paecilomyces sp.)是一种广谱性的虫生真菌,其寄主范围跨同翅目、鳞翅目、鞘翅目和蜱螨目11种昆虫和螨类。其致病力受到许多环境因素的影响,除了常见的温度、湿度、pH值、培养基种类以外,其寄主昆虫种类也是影响其致病力的一个重要因素。该菌侵染各种寄主昆虫以后,分离出来的各菌株致病力明显不同,用6个菌株及其代谢产物进行的侵染和杀虫试验表明,菌株间的致病力和杀虫活性有显著差异。其中PA9032菌株的侵染力最强,其代谢产物杀虫活性最强,24小时杀山楂叶螨的效果为70.0%。此结果表明,用同一种微生物侵染不同昆虫后,可以分离到致病力不同菌株,此法有可能发展成为菌株筛选的一种新方法。更多还原

【Abstract】 The results demonstrated that Paeciloyces sp.is an entomopathogenic fungus with large scale of host.It can parasite over 11 species of insects in Homopteara,Coleoptera and Acarina.The pathogenicity was effected by many environmental factors,i.e.temperatrue,moisty,pH value and medium.Besides of above factors,the species of insects as host can effect the pathogenicity of the fungus significantly.The isolates from different Species of insects were used to infect Myzus per sices.The results showed that there were significant variation in pathogenicity.Among them,the isolate PA9032 was most toxic to insects and red mite.The mortality of hawthorn spider was 70.0%at 24 hours.The method weused in the study may be developed as a new method for screening effective strains.更多还原