Expression and function analysis of metallothionein in the testis of Portunus trituberculatus exposed to Cadmium

【作者】 向东方； 盛樟； 竺俊全；

【机构】 宁波大学海洋学院；

【摘要】 <正>Metallothioneins(MTs) possess a unique molecular structure that provides metal-binding and redox capabilities.These capabilities include the maintenance of metal equilibria that protect against heavy metals(especially Cadmium) and oxidative damage.Past studies have focused on the function of MTs in vertebrates.However,the functions of MTs during spermiogenesis in invertebrates remain unclear.In order to investigate the function of MTs during spermiogenesis in P.trituberculatus,we used RT-PCR and RACE to identify two MT complete cDNA sequences in the total RNA from the P.trituberculatus testis.The 450bp MT-1 cDNA consists of a 77 bp 5’ untranslated region,a 196 bp 3’ untranslated region,and a 177 bp open reading frame that encodes 58 amino acids including 19 cysteines.The 581bp MT-2 cDNA consists of 73 bp 5’ untranslated region,a 328 bp 3’ untranslated region,and a 180 bp open reading frame that encodes 59 amino acids including 18 cysteines.MT-1 and MT-2 of P. trituberculatus more closely resemble invertebrate(especially crab) MT homologues than vertebrate MT homologues as indicated by protein alignment comparisons and phylogenetic tree analysis.MT-1 and MT-2 were detected in the heart,testis,muscle,hepatopancreas,and gill of P. trituberculatus by tissue expression analysis.In addition,MT-1 and MT-2 are present during the entire process of spermiogenesis in P.trituberculatus as indicated by H&E staining and in situ hybridization.MT-1 and MT-2 expression levels significantly increase after Cadmium(Cd) exposure as measured by real-time quantitative PCR analysis.Therefore,we suggest that MT-1 and MT-2 perform important functions in spermiogenesis and testis detoxification in P. trituberculatus.更多还原

【Abstract】 Metallothioneins(MTs) possess a unique molecular structure that provides metal-binding and redox capabilities.These capabilities include the maintenance of metal equilibria that protect against heavy metals(especially Cadmium) and oxidative damage.Past studies have focused on the function of MTs in vertebrates.However,the functions of MTs during spermiogenesis in invertebrates remain unclear.In order to investigate the function of MTs during spermiogenesis in P.trituberculatus,we used RT-PCR and RACE to identify two MT complete cDNA sequences in the total RNA from the P.trituberculatus testis.The 450bp MT-1 cDNA consists of a 77 bp 5’ untranslated region,a 196 bp 3’ untranslated region,and a 177 bp open reading frame that encodes 58 amino acids including 19 cysteines.The 581bp MT-2 cDNA consists of 73 bp 5’ untranslated region,a 328 bp 3’ untranslated region,and a 180 bp open reading frame that encodes 59 amino acids including 18 cysteines.MT-1 and MT-2 of P. trituberculatus more closely resemble invertebrate(especially crab) MT homologues than vertebrate MT homologues as indicated by protein alignment comparisons and phylogenetic tree analysis.MT-1 and MT-2 were detected in the heart,testis,muscle,hepatopancreas,and gill of P. trituberculatus by tissue expression analysis.In addition,MT-1 and MT-2 are present during the entire process of spermiogenesis in P.trituberculatus as indicated by H&E staining and in situ hybridization.MT-1 and MT-2 expression levels significantly increase after Cadmium(Cd) exposure as measured by real-time quantitative PCR analysis.Therefore,we suggest that MT-1 and MT-2 perform important functions in spermiogenesis and testis detoxification in P. trituberculatus.更多还原